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Human metaphase II oocytes with narrow perivitelline space have poor fertilization, developmental, and pregnancy potentials

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To analyze the fertilization, developmental, and pregnancy potentials in oocytes with narrow perivitelline space.

Methods

Perivitelline space (PVS) of oocytes was evaluated at the time of ICSI, and those without sufficient PVS were judged as oocytes with narrow PVS (NPVS oocytes), and those with sufficient PVS formation were judged as oocytes with non-narrow PVS (non-NPVS oocytes). The analysis included 634 NPVS oocytes from 278 cycles and 12,121 non-NPVS oocytes from 1698 cycles. The fertilization and developmental potentials of NPVS and non-NPVS oocytes were compared by calculating odds ratios using a mixed-effects logistic regression model. We also compared the embryo transfer outcomes of those used for single vitrified-warmed blastocyst transfer after developing into the blastocyst stage.

Results

NPVS oocytes had higher odds ratios for degeneration (adjusted odds ratio [aOR], 1.555; 95% confidence interval [CI], 1.096–2.206; p = 0.0133) and 0PN (aOR, 1.387; 95% CI, 1.083–1.775; p = 0.0095), resulting in a lower 2PN rate (aOR, 0.761; 95% CI, 0.623–0.929; p = 0.0072). Even embryos with confirmed 2PN had lower odds ratios for cleavage (aOR, 0.501; 95% CI, 0.294–0.853; p = 0.0109) and blastocyst development (Gardner criteria; CC–AA) rates (aOR, 0.612; 95% CI, 0.476–0.788; p = 0.0001). Blastocysts developed from NPVS oocytes had significantly lower odds ratios for clinical pregnancy (aOR, 0.435; 95% CI, 0.222–0.854; p = 0.0156) than those developed from non-NPVS oocytes.

Conclusions

Oocytes with NPVS have low fertilization and developmental potential, as well as low likelihood of pregnancy.

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Acknowledgements

We thank the staff of Takahashi Women’s Clinic. We also would like to thank the Chiba University Academic English consultation service for the English language review. Furthermore, we would like to show our greatest appreciation to Dr. Yuki Shiko at the Clinical Research Center, Chiba University Hospital for advice on statistical analysis.

Funding

The authors have no relevant financial or non-financial interests to disclose.

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Authors and Affiliations

  1. Takahashi Women’s Clinic, 18-14-6F Shinmachi, Chuo-ku, Chiba, 260-0028, Japan

    Masashi Shioya, Miki Okabe-Kinoshita, Maki Fujita & Keiichi Takahashi

  2. Department of Reproductive Medicine, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan

    Masashi Shioya & Tatsuya Kobayashi

  3. Department of Regulatory Science, Fujita Health University, Haneda Innovation City Zone A, 1-1-4 Haneda Airport, Ota-ku, Tokyo, 144-0041, Japan

    Tatsuya Kobayashi

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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Masashi Shioya, Miki Okabe-Kinoshita, Tatsuya Kobayashi, Maki Fujita, and Keiichi Takahashi. The first draft of the manuscript was written by Masahi Shioya, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Masashi Shioya.

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Shioya, M., Okabe-Kinoshita, M., Kobayashi, T. et al. Human metaphase II oocytes with narrow perivitelline space have poor fertilization, developmental, and pregnancy potentials. J Assist Reprod Genet (2024). https://doi.org/10.1007/s10815-024-03084-y

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