Abstract
The nucleotide sequence of a mitochondrial genome of the pulmonate gastropod molluscCepaea nemoralis has been determined. Contained within the 14,100 basepairs (bp) are the two ribosomal RNA genes and 13 protein coding genes typical of metazoan mitochondrial genomes. TheCepaea mtDNA does contain a gene for ATPase subunit 8, like the clausiliid pulmonate,Albinaria, and the chiton,Katharina, but unlike the bivalve mollusc,Mytilus. The mitochondrial genetic code ofCepaea is proposed to be the same as that ofMytilus, Katharina, andDrosophila. Only 14 putative tRNA genes are presented, although there is sufficient unassigned sequence to encode the remainder of the expected total of 22 tRNA genes. These 14 tRNA genes are a mixture of standard cloverleaf structures and nonstandard structures containing TV replacement loops as seen in nematode and mosquito mitochondrial genomes. If the eight unidentified tRNA genes are indeed present, very little unassigned sequence would remain to serve as a control region. Genes are transcribed from both strands of the molecule. Base composition is the least biased for any reported animal mitochondrial genome and is also very little skewed between strands using measures independent of base composition. TheCepaea mitochondrial gene order is quite unlike that of any other reported metazoan mtDNA, with the exception of the recently reported partial sequences ofAlbinaria. No gene bound-aries are shared among all the reported molluscan taxa, demonstrating a complete lack of conservation of mitochondrial gene order across the phylum Mollusca.
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Anderson S, Bankier AT, Barrell BG, de Brujin MHL, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG (1981) Sequence and organization of the human mitochondrial genome. Nature 290:457–465
Bibb MJ, van Etten RA, Wright CT, Walberg MW, Clayton DA (1981) Sequence and gene organization of mouse mitochondrial DNA. Cell 26:167–180
Boore JL, Brown WM (1994) Complete DNA sequence of the mitochondrial genome of the black chiton,Katharina tunicata. Genetics 138:423–443
Brown WM (1985) The mitochondrial genome of animals. In: MacIntyre RJ (ed) Molecular evolutionary genetics. Plenum Press, New York
Clary DO, Wolstenholme DR (1985a) The mitochondrial DNA molecule ofDrosophila yakuba: nucleotide sequence, gene organisation, and genetic code. J Mol Evol 22:252–271
Clary DO, Wolstenholme DR (1985b) The ribosomal RNA genes ofDrosophila mitochondrial DNA. Nucleic Acids Res. 13:4029–4045
Clayton DA (1992) Transcription and replication of animal mitochondrial DNAs. Int Rev Cytol 141:217–232
Garey JR, Wolstenholme DR (1989) Platyhelminth mitochondrial DNA: evidence for early evolutionary origin of a tRNAser AGN that contains a dihydrouridine arm replacement loop, and of serine-specifying AGA and AGG codons. J Mol Evol 28:374–387
Henikoff S (1984) Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
Higgins DG, Bleasby AJ, Fuchs R (1992) CLUSTALV—improved software for multiple sequence alignment. Comput Appl Biosci 8:189–191
Hoffmann RJ, Boore JL, Brown WM (1992) A novel mitochondrial genome organization for the Blue Mussel,Mytilus edulis. Genetics 131:397–412
Jacobs HT, Elliott DJ, Math VB, Farquharson S (1988) Nucleotide sequence and gene organization of sea urchin mitochondrial DNA. J Mol Biol 202:185–217
Janke A, Pääbo S (1993) Editing of a tRNA anticodon in marsupial mitochondria changes its codon recognition. Nucleic Acids Res 21:1523–1525
Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105–132
Lecanidou R, Douris V, Rodakis GC (1994) Novel features of metzoan mtDNA revealed from sequence analysis of three mitochondrial DNA segments of the land snailAlbinaria turrita (Gastropoda: Clausiliidae). J Mol Evol 38:369–382
Mitchell SE, Cockburn AF, Seawright JA (1993) The mitochondrial genome ofAnopheles quadrimaculatus species A: complete nucleotide sequence and gene organization. Genome 36:1058–1073
Neefs JM, Vandepeer Y, Derijk P, Goris A, Dewachter R (1991) Compilation of small ribosomal subunit RNA sequences. Nucleic Acids Res 19:1987–2015
Okimoto R, Chamberlin HM, Macfarlane JL, Wolstenholme DR (1991) Repeated sequence sets in mitochondrial DNA molecules of root knot nematodes (Meloidogyne): nucleotide sequences, genome location and potential for host race identification. Nucleic Acids Res 19:1619–1626
Okimoto R, Macfarlane JL, Clary DO, Wolstenholme DR (1992) The mitochondrial genomes of two nematodes,Caenorhabditis elegans andAscaris suum. Genetics 130:471–498
Perna NT, Kocher TD (1995) Patterns of compositional bias and skew at four-fold degenerate sites of animal mitochondrial genomes. J Mol Evol (in press)
Roe BA, Ma D-P, Wilson RK, Wong JF-H (1985) The complete nucleotide sequence of theXenopus laevis mitochondrial genome. J Biol Chem 260:9759–9774
Runnegar B, Pojeta J (1985) Origin and diversification of the Mollusca. In: Trueman RE, and Clarke MR (eds) The mollusca, Vol 10, Evolution. Academic Press, New York, p 1–57
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Sankoff D, Leduc G, Antoine N, Paquin B, Lang BF, Cedergren R (1992) Gene order comparisons for phylogenetic inference: evolution of the mitochondrial genome. Proc Natl Acad Sci USA 89:6575–6579
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Stine OC (1989)Cepaea nemoralis from Lexington, Virginia: the isolation and characterization of their mitochondrial DNA, the implications for their origin and climatic selection. Malacologia 30:305–315
Terrett JA (1992) The mitochondrial genome ofCepaea nemoralis. Unpublished PhD thesis, University of Nottingham
Terrett JA, Miles S, Thomas RH (1994) The mitochondrial genome ofCepaea nemoralis (Gastropoda: Stylommatophora): gene order, base composition and heteroplasmy. Nautilus Suppl 2:79–84
Vieira J, Messing J (1987) Production of single stranded plasmid DNA. Methods Enzymol 153:3
Wolstenholme DR (1992) Animal mitochondrial DNA: structure and evolution. Int Rev Cytol 141:173–216
Wolstenholme DR, Macfarlane JL, Okimoto R, Clary DO, Wahleithner JA (1987) Bizarre tRNA's inferred from DNA sequences of mitochondrial genomes of nematode worms. Proc Natl Acad Sci USA 84:1324–1328
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Terrett, J.A., Miles, S. & Thomas, R.H. Complete DNA sequence of the mitochondrial genome ofCepaea nemoralis (Gastropoda: Pulmonata). J Mol Evol 42, 160–168 (1996). https://doi.org/10.1007/BF02198842
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DOI: https://doi.org/10.1007/BF02198842