Abstract
The effects of small bowel transplantation (SBTx) on absorptive function are unknown. Preliminary experiments showed a decrease in absorption of glutamine. Our aim was to determine mechanisms of decreased ileal transport of glutamine utilizing a model of intestinal autotransplantation. Seven dogs were studied before and after a model of jejunoileal autotransplantation.In vivo absorption experiments were performed before and two and eight weeks postoperatively with an electrolyte solution containing glutamine (20 mM).In vitro glutamine transport was studied using brush-border membrane vesicles (BBMV) prepared from ileal mucosa obtained from six other dogs and compared to a controls.In vivo net absorptive flux of glutamine decreased at two weeks but returned toward baseline by eight weeks (P=0.06). Transport of glutamine into BBMVs was decreased at two weeks and remained decreased at eight weeks.\(K_{\max }^{Na_ + } \), a measure of carrier affinity was unchanged but\(V_{\max }^{Na_ + } \), a function of the number of transporter was decreased at two and eight weeks. Glucose transport was unchanged. It is concluded that jejunoileal autotransplantation decreases ileal absorption of glutamine by a decrease in carrier-mediated transport of glutamine.
Similar content being viewed by others
References
Abu-Elmagd K, Todo S, Tzakis AG, Reyes J, Nour B, Furukawa H, Fung JJ, Demetris A, Starzl TE: Three years clinical experience with intestinal transplantation. J Am Coll Surg 179:385–400, 1994
Todo S, Tzakis AG, Abu-Elmagd K, Reyes J, Fung JJ, Casavilla A, Nakamura K, Yagihashi A, Jain A, Murase N, Iwaki Y, Demetris AJ, van Thiel D, starzl TE: Cadaveric small bowel and small bowel liver transplantation in humans. Transplantation 53:369–376, 1992
Sarr MG, Kelly KA: Myoelectric activity of the autotransplanted canine jejunoileum. Gastroenterology 81:303–310, 1981
Thompson JS, Rose SG, Spanta AD, Quigley EMM: The long-term effect of jejunoileal autotransplantation on intestinal function. Surgery 110:394–401, 1992
Herkes SM, Smith CD, Sarr MG: Jejunal responses to absorptive and secretory stimuli in the neurally isolated jejunumin vivo. Surgery 116:576–586, 1994
Oishi AJ, Sarr MG: Intestinal transplantation: Effects on ileal enteric absorptive physiology. Surgery 117:545–553, 1995
Sarr MG, Duenes JA, Walters AM: Jejunal and ileal absorptive function after a model of canine jejunoileal autotransplantation. J Surg Res 51:233–239, 1991
Hakim NS, Walters AM, Zinsmeister AR, Sarr MG: Net absorption of water, electrolytes, glucose, and folate from thein vivo, neurally isolated canine jejunum. Surgery 110:394–401, 1992
Sarr MG, Walters AM, Benson JT, Zinsmeister AR: Absorption of simple nutrients from thein vivo neurally isolated canine jejunum and ileum: A model of intestinal transplantation. Surgery 115:578–587, 1994
Oishi AJ, Inoue Y, Souba WW, Sarr MG: Selective downregulation of glutamine transport after jejunoileal autotransplantation. Gastroenterology 108:A1235, 1995
Windmueller HG: Glutamine utilization by the small intestine. Adv Enzymol 53:202–231, 1982
Souba WW, Herskowitz K, Salloum RM, Chen MK, Austgen TR: Gut glutamine metabolism. J Parenteral Enteral Nutr 14(suppl):45S-50S, 1990
Salloum RM, Copeland EM, Souba WW: Brush border transport of glutamine and other substrates during spesis and endotoxemia. Ann Surg 213:401–410, 1991
Stevens BR, Kaunitz J, Wright EM: Intestinal transport of amino acids and sugars. Advances using membrane vesicles. Annu Rev Physiol 417:417–433, 1984
Sarr MG, Duenes JA, Tanaka M: A model of jejunoilealin vivo neural isolation of the entire jejunoileum: Transplantation and the effects on intestinal motility. J Surg Res 47:266–272, 1989
Sarr MG, Duenes JA, Tanaka M: Jejunoileal autotransplantation: Effects on small intestinal motility. Surg Forum 38:160–162, 1987
Barbezat GO: Triple lumen perfusion of the canine jejunum. Gastroenterology 79:1243–1245, 1980
Cooper H, Levitan R, Fordtran JS, Ingelfinger FJ: Method for studying absorption of water and solute from the human intestine. Gastroenterology 50:1–7, 1966
Stevens BR, Wright SH, Hirayama BS, Ross HJ, Gunther R, Nord E, Kippen L, Wright EM: Organic and inorganic solute transport in renal and intestinal membrane vesicles preserved in liquid nitrogen. Membr Biochem 4:271–281, 1982
Mircheff AK, Wright EM: Analytical isolation of plasma membranes of intestinal epithelial cells, identification of Na-K ATPase and the distribution of enzyme activities. J Membr Biol 28:309–333, 1976
Salloum RM, Stevens BR, Souba WW: Adaptive regulation of brush border amino acid transport in a chronic excluded jejunal limb. Am J Physiol 261:G22-G27, 1991
Klimberg VS, Souba WW: The importance of intestinal glutamine metabolism in maintaining a healthy gastrointestinal tract and supporting the body's response to injury and illness. Surg Annu 22:61–76, 1990
Souba WW, Herskowitz K, Klimberg VS, Salloum RM, Plumley DA, Flynn TC, Copeland EM: The effects of sepsis and endotoxemia on gut glutamine metabolism. Ann Surg 211:543–550, 1990
Sarr MG, Siadati MR, Bailey J, Lucas DL, Roddy DR, Duenes JA: Neural isolation of the jejunoileum: Effect on tissue morphometry, mucosal disacchariase activity, and tissue peptide content. J Surg Res 61:416–424, 1996
McCord JM: Oxygen derived free radicals in post ischemic tissue injury. N Engl J Med 312:159–163, 1985
Author information
Authors and Affiliations
Additional information
Supported in part by NIH DK39337 (M.G.S.), NIH CA45327 (W.W.S.), and the Mayo Foundation.
Rights and permissions
About this article
Cite this article
Oishi, A.J., Inoue, Y., Souba, W.W. et al. Alterations in carrier-mediated glutamine transport after a model of canine jejunal autotransplantation. Digest Dis Sci 41, 1915–1924 (1996). https://doi.org/10.1007/BF02093590
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02093590