Skip to main content
SpringerLink
Log in

Estimates of free and bound indole-3-acetic acid and zeatin levels in relation to regulation of apical dominance and tiller release in oat shoots

  • Published:
Journal of Plant Growth Regulation Aims and scope Submit manuscript

Abstract

Oat stem segments containing quiescent lateral (tiller) buds during times of strong apical dominance, and growing buds released from this inhibition, were collected for analysis of native auxin and cytokinins. Free IAA and IAA conjugates were determined by a14C-IAA and14C-IBA double isotope dilution assay. Free zeatin (Z), zeatin riboside (Z-r), and their glucoside conjugates were purified from butanol-soluble fractions by means of a cellulose phosphate exchanger and thin-layer chromatography. Hormones were analyzed by gas chromatography and mass spectrometry (GC-MS). Results of these analyses indicate that changes in free and bound IAA within the stem do not correlate well with the release of tiller buds (as brought about by decapitation, gravistimulation, or the emergence of the inflorescence). However, increases in Z-r levels are well correlated with tiller release. The glucoside conjugate of Z-r may act as a storage form of cytokinin in quiescent tiller buds. In light of these results, we find that the auxin-cytokinin ratio in oat stem segments is shifted during tiller

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

IAA:

indole-3-acetic acid

Z:

zeatin

BA:

benzyl adenine

Z-r:

zeatin riboside

MeIAA:

methyl ester of indole-3-acetic acid

Me-IBA:

methyl ester of indole-3-butyric acid

ABA:

abscissic acid

NPD:

nitrogen-phosphorus detector for gas chromatograph

HPLC:

high-performance liquid chromatography

GC:

gas chromatography

MS:

mass spectrometry

TLC:

thin-layer chromatography

References

  • Bandurski RS, Schulze A (1977) Concentration of indole-3-acetic acid and its derivatives in plants. Plant Physiol 60:211–213

    PubMed  CAS  Google Scholar 

  • Bandurski RS, Schulze A (1974) Concentration of indole-3-acetic acid and its esters inAvena andZea. Plant Physiol 54:257–262

    Article  PubMed  CAS  Google Scholar 

  • Bieleski RL (1964) The problem of halting enzyme action when extracting plant tissues. Anal Biochem 9:431–442

    Article  PubMed  CAS  Google Scholar 

  • Cohen JD, Shultze A (1981) Double-standard isotope dilution assay. I. Quantitative assay of indole-3-acetic acid. Anal Biochem 112:249–257

    Article  PubMed  CAS  Google Scholar 

  • Dekhuijzen HM (1980) The occurrence of free and bound cytokinins in clubroots andPlasmodiophora brassicae infected turnip tissue cultures. Physiol Plant 49:169–176

    Article  Google Scholar 

  • Hangarter RP, Good NE (1981) Evidence that IAA conjugates are slow-release sources of free IAA in plant tissues. Plant Physiol 68:1424–1427

    PubMed  CAS  Google Scholar 

  • Harrison MA, Kaufman PB (1982) Does ethylene play a role in the release of lateral buds (tillers) from apical dominance in oats? Plant Physiol 70:811–814

    PubMed  CAS  Google Scholar 

  • Harrison MA (1982) The hormonal regulation of lateral bud (tiller) development in oats (Avena sativa L.). PhD Thesis. University of Michigan, Ann Arbor, Michigan, USA

    Google Scholar 

  • Harrison MA, Kaufman PB (1980) Hormonal regulation of lateral bud (tiller) release in oats (Avena saliva L.). Plant Physiol 66:1123–1127

    PubMed  CAS  Google Scholar 

  • Jablanovic M, Neskovic M (1977) Changes in endogenous level of auxins and cytokinins in axillary buds ofPisum sativum L. in relation to apical dominance. Biol Plant 19:34–39

    Article  CAS  Google Scholar 

  • Kuhnle JA, Fuller G, Corse J, MacKey BE (1977) Antisenescent activity of natural cytokinins. Physiol Plant 41:14–21

    Article  CAS  Google Scholar 

  • Morris RO, Akiyoshi DE (1983) Dimethylallylpyrophosphate-5′ AMP transferase activity and cytokinin levels in mutated crown gall tumors. Plant Physiol 72:S-3

    Google Scholar 

  • Tucker DJ (1977) Apical dominance in the ‘Rogue’ tomato. Ann Bot 41:181–190

    Google Scholar 

  • Wickson M, Thimann KV (1958) The antagonism of auxin and kinetin in apical dominance. Physiol Plant 11:62–74

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Botany and Cellular and Molecular Biology, University of Michigan, 48109, Ann Arbor, Michigan, USA

    Marcia A. Harrison & Peter B. Kaufman

Authors
  1. Marcia A. Harrison
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Peter B. Kaufman
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by a grant (NAGW-34) from the National Aeronautics and Space Administration.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Harrison, M.A., Kaufman, P.B. Estimates of free and bound indole-3-acetic acid and zeatin levels in relation to regulation of apical dominance and tiller release in oat shoots. J Plant Growth Regul 2, 215–223 (1983). https://doi.org/10.1007/BF02042250

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02042250

Keywords

Search

Navigation