Abstract
Albino (Sprague-Dawley) and pigmented (Norwegian Brown) male rats were exposed to 2,5-hexanediol (H; 1%) in their drinking water for 5 or 8 weeks, respectively. Half of the rats of each strain were housed in light (average 30 cd/cm2 inside cage, 12 h/day); the other half was kept in constant darkness. Control groups were studied in parallel under identical conditions but without H. Electrophysiological recordings were made 2–5 days and 13 weeks after the end of the exposure to H. Alterations in the visual system, as measured by electroretinography and visual evoked response, were found in groups of albino rats exposed to H and/or light. The pupillary diameter was enlarged in the albino group exposed to both H and light. Among the pigmented rats, alterations were recorded only in the visual evoked response of the H exposed groups. The results demonstrate that simultaneous exposure to H and light can lead to alterations in visual function that are more severe than those induced by each agent alone, and may exceed a simple summation.
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References
Abdel-Rahman MS, Saladin JJ, Bohman CE, Couri D (1978) The effect of 2-hexanone and 2-hexanone metabolites on pupillomotor activity and growth. Am Ind Hyg Assoc J 39: 94–99
Altenkirch H, Mager J, Stoltenburg G, Helmbrecht J (1977) Toxic polyneuropathies after sniffing a glue thinner. J Neurol 214: 137–152
Altenkirch H, Wagner HM, Stoltenburg-Didinger G, Steppat R (1982) Potentiation of hexacarbon-neurotoxicity by methyl-ethyl-ketone (MEK) and other substances: clinical and experimental aspects. Neurobehav Toxicol Teratol 4: 623–627
Blain L, Mergler D (1986) La dyschromatopsie chez des personnes exposées professionnellement aux solvants organiques. J Fr Ophthalmol 9: 127–133
Boekelheide K (1987) 2,5-Hexanedione alters microtubule assembly. I. Testicular atrophy, not nervous system toxicity, correlates with enhanced tubulin polymerization. Toxicol Appl Pharmacol 88: 370–382
Boekelheide K, Neely MD, Sioussat TM (1989) The Sertoli cell cytoskeleton: a target for toxicant-induced germ cell loss. Toxicol Appl Pharmacol 101: 373–389
Bäckström B, Collins VP (1987) Cytoskeletal changes in axons of rats exposed to 2,5-hexanediol, demonstrated using monoclonal antibodies. Neurotoxicology 8: 85–96
Bäckström B, Dumanski JP, Collins VP (1990) The effects of 2,5-hexanedione on the retina of albino rats. Neurotoxicology 11: 47–56
Bäckström B, Nylén P, Hagman M, Johnson A-C, Höglund G, Collins VP (1993) Effect of exposure to 2,5-hexanediol in light or darkness on the retina of albino and pigmented rats. I. Morphology. Arch Toxicol 67: 277–283
Chaitin MH, Burnside B (1989) Actin filament polarity at the site of rod outer segment disc morphogenesis. Invest Ophthalmol Vis Sci 30: 2461–2469
Chang YC (1990) Patients withn-hexane induced polyneuropathy: a clinical follow up. Br J Ind Med 47: 485–489
Cody RP, Smith JK (1987) Applied statistics and the SAS programming language, 2nd edn. Elsevier Science Publishing Co, New York
Gobba F, Galassi C, Imbriani M, Ghittori S, Candela S, Cavalleri A (1991) Acquired dyschromatopsia among styrene-exposed workers. J Occup Med 33: 761–765
Hansson HA (1970) Ultrastructural studies on rat retina damaged by visible light. Virchows Arch Abt B, Zellpathol 6: 247–262
Hebel R, Stromberg MW (1986) Anatomy and embryology of the laboratory rat. BioMed Verlag, Wörthsee, pp 218–223
Hennekes R (1989) Klinische Elektroretinographie. Fortschr Ophthalmol 86: 146–150
Herskowitz A, Ishii N, Schaumburg H (1971) V-hexane neuropathy. A syndrome occurring as a result of industrial exposure. N Engl J Med 285: 82–85
Hirata M (1990) Reduced conduction function in central nervous system by 2,5-hexanedione. Neurotoxicol Teratol 12: 623–626
Jones HB, Cavanagh JB (1982a) The early evolution of neurofilamentous accumulations due to 2,5-hexanediol in the optic pathways of the rat. Neuropathol Appl Neurobiol 8: 289–301
Jones HB, Cavanagh JB (1982b) Recovery from 2,5-hexanediol intoxication of the retinotectal tract of the rat. An ultrastructural study. Acta Neuropathol 58: 286–290
Mergler D, Blain L (1987) Assessing colour vision loss among solventexposed workers. Am J Ind Med 12: 195–203
Mergler D, Bélanger S, De Grosbois S, Vachon N (1988) Chromai focus of acquired chromatic discrimination loss and solvent exposure among printshop workers. Toxicology 49: 341–348
Monaco S, Wongmongkolrit T, Shearson CM, Patton A, Schaetzle B, Autilio-Gambetti L, Gambetti P, Sayre LM (1990) Giant axonopathy characterized by intermediate location of axonal enlargements and acceleration of neurofilament transport. Brain Res 519: 73–81
Neely M, Boekelheide K (1988) Sertoli cell processes have axoplasmic features: an ordered microtubule distribution and an abundant high molecular weight microtubule-associated protein (cytoplasmic dynein). J Cell Biol 107: 1767–1776
Noell WK, Walker VS, Kang BS, Berman S (1966) Retinal damage by light in rats. Invest Ophthalmol 5: 450–473
Nylén P, Ebendal T, Eriksdotter-Nilsson M, Hansson T, Henschen A, Johnson A-C, Kronevi T, Kvist U, Sjöstrand NO, Höglund G, Olson L (1989) Testicular atrophy and loss of nerve growth factorimmunoreactive germ cell line in rats exposed to n-hexane and a protective effect of simultaneous exposure to toluene or xylene. Arch Toxicol 63: 296–307
O'Donoghue JL, Krasavage WJ, Terhaar CJ (1978) Toxic effects of 2,5-hexanedione. Toxicol Appl Pharmacol 45: 269
Pasternak T, Flood DG, Eskin TA, Merigan WH (1985) Selective damage to large cells in the cat retinogeniculate pathway by 2,5-hexanedione. J Neurosci 5: 1641–1652
Paulson G, Waylonis G (1976) Polyneuropathy due to n-hexane. Arch Int Med 136: 880–882
Pecci Saavedra J, Pellegrino de Iraldi A (1976) Retinal alterations induced by continuous light in immature rats. I. Fine structure and electroretinography. Cell Tissue Res 166: 201–211
Raitta C, Seppäläinen AM, Huuskonen MS (1978) N-hexane maculopathy in industrial workers. Albrecht von Graefes Arch Klin Exp Ophthalmol 209: 99–110
Rapp LM, Williams TP (1979) Damage to the albino rat retina produced by low intensity light. Photochem Photobiol 29: 731–733
Rapp LM, Williams TP (1980) The role of ocular pigmentation in protecting against retinal light damage. Vision Res 20: 1127–1131
Rebert CS, Sorenson SS (1983) Concentration-related effects of hexane on evoked responses from brain and peripheral nerve of the rat. Neurobehav Toxicol Teratol 5: 69–76
Reuter JH, Hobbelen JF (1977) The effect of continuous light exposure on the retina in albino and pigmented rats. Physiol Behav 18: 939–944
Seppäläinen AM, Raitta C, Huuskonen MS (1979) Hexane induced changes in visual evoked potentials and electroretinograms of industrial workers. EEG Clin Neurophysiol 47: 492–498
Spencer PS, Schaumburg HH, Sabri MI, Veronesi B (1980) The enlarging view of hexacarbon neurotoxicity. CRC Crit Rev Toxicol 7: 279–356
Teir H (1988) An ophthalmological study on workers with long-term occupational exposure to industrial solvents. Accademic dissertion from the Department of Ophthalmology, University of Helsinki, Helsinki
Wang JD, Chang YC, Kao KP, Huang CC, Lin CC, Yeh WY (1986) An outbreak ofn-hexane induced polyneuropathy among press proofing workers in Taipei. Am J Ind Med 10: 111–118
Williams RA, Howard AG, Williams TP (1985) Retinal damage in pigmented and albino rats exposed to low levels of cyclic light following a single mydriatic treatment. Curr Eye Res 4: 97–102
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Nylén, P., Bäckström, B., Hagman, M. et al. Effect of exposure to 2,5-hexanediol in light or darkness on the retina of albino and pigmented rats. II. Electrophysiology. Arch Toxicol 67, 435–441 (1993). https://doi.org/10.1007/BF01977406
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DOI: https://doi.org/10.1007/BF01977406