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Enzyme specific kinetics of 1,2-epoxybutene-3 in microsomes and cytosol from livers of mouse, rat, and man

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Abstract

Kinetics of the metabolism of 1,2-epoxybutene-3 (butadiene monoxide) were investigated in liver fractions of mouse, rat, and man. In these species similar enzyme characteristics were found. In microsomes, no NADPH-dependent metabolism of butadiene monoxide was detectable. Epoxide hydrolase activity was found only in microsomes. The Vmax [nmol butadiene monoxide/(mg protein x min)] was 19 in mouse, 17 in rat, and 14 in man and the apparent Km (mmol butadiene monoxide/l incubate) was 1.5 in mouse, 0.7 in rat, and 0.5 in man. Glutathione S-transferase activity was found in cytosol only, revealing first order kinetics in the measured range. The ratio Vmax/Km [(nmol butadiene monoxide x l)/(mg protein × min × mmol of butadiene monoxide)] was 15 in mouse, 11 in rat, and 8 in man. The data obtained were used to extrapolate on the total rate of butadiene monoxide metabolism for each species in vivo: it was calculated to be 1.3 times higher in mice and 2.3 times lower in man compared to rats, when corrected for body weight.

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Authors and Affiliations

  1. GSF-Institut für Toxikologie Ingolstädter Landstrasse 1, W-8042, Neuherberg, Federal Republic of Germany

    P. E. Kreuzer, W. Kessler & J. G. Filser

  2. Klinikum Großhadern, Ludwig-Maximilians-Universität München, Marchioninistrasse 15, W-8000, München 70, Federal Republic of Germany

    H. F. Welter

  3. Institut für Gerichtsmedizin, Ludwig-Maximilians-Universität München, Frauenlobstrasse 7 a, W-8000, München 2, Federal Republic of Germany

    C. Baur

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  1. P. E. Kreuzer
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  4. C. Baur
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  5. J. G. Filser
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Kreuzer, P.E., Kessler, W., Welter, H.F. et al. Enzyme specific kinetics of 1,2-epoxybutene-3 in microsomes and cytosol from livers of mouse, rat, and man. Arch Toxicol 65, 59–67 (1991). https://doi.org/10.1007/BF01973504

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  • DOI: https://doi.org/10.1007/BF01973504

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