Skip to main content
SpringerLink
Log in

Influence of lithium upon the intracellular potential of frog skin epithelium

  • Published:
The Journal of Membrane Biology Aims and scope Submit manuscript

Summary

The effect of Li upon the intracellular potential of frog skin (Rana esculenta) was investigated. In the range between 1 and 25mm Li in the epithelial bathing solution, a semilogarithmic linear relationship between [Li] and intracellular potential under short circuit conditions was obtained. The intracellular potential at all [Li] is quantitatively sufficient to explain the previously reported accumulation of Li in the intracellular space of the frog skin epithelium (Leblanc, G. 1972.Pfluegers Arch. 337:1) on the basis of a passive entrance step at the outer border. A reduction of the intracellular potential by Li is also observed in the presence of 6mm Na in the epithelial bathing solution. Consequences regarding the mechanism of uptake of Na across the outer border of the frog skin are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aceves, J., Erlij, D. 1971. Sodium transport across the isolated epithelium of the frog skin.J. Physiol. (London) 212:195

    Google Scholar 

  • Bentley, P.J. 1968. Amiloride: A potent inhibitor of sodium transport across the toad bladder.J. Physiol. (London) 195:317

    Google Scholar 

  • Biber, T.U.L., Curran, P.E. 1970. Direct measurement of uptake of sodium at the outer surface of the frog skin.J. Gen. Physiol. 56:83

    PubMed  Google Scholar 

  • Biber, T.U.L., Sanders, U.L. 1973. Influence of transepithelial potential difference on the sodium uptake at the outer surface of the isolated frog skin.J. Gen. Physiol. 61:529

    PubMed  Google Scholar 

  • Candia, O.A., Chiarandini, D.J. 1973. Transport of lithium and rectification by frog skin.Biochim. Biophys. Acta 307:578

    PubMed  Google Scholar 

  • Cirne, B., Lindemann, B. 1975. Passive nature of Li and Na transport through the Na-selective membrane of frog skin. p. 66. (Abstr.) V. Internat. Biophys. Congress, Copenhagen

  • Dörge, A., Gehring, K., Nagel, W., Thurau, K. 1974. Location of sodium in frog skin by electron microprobe analysis.Naunyn-Schmiedeberg's Arch. Pharmacol. 281:271

    Article  Google Scholar 

  • Dörge, A., Nagel, W. 1970. Effect of amiloride on sodium transport in frog skin. II. Sodium transport pool and unidirectional fluxes.Pfluegers Arch. 321:91

    Article  Google Scholar 

  • Eigler, J., Kelter, J., Renner, E. 1967. Wirkungscharakteristika eines neuen Acylguanidins-Amilorid-HCl (MK 870) an der isolierten Haut von Amphibien.Klin. Wochenschr. 14:737

    Article  Google Scholar 

  • Hansen, H.H., Zerahn, K. 1964. Concentration of lithium, sodium and potassium in epithelial cells of the isolated frog skin during active transport of lithium.Acta Physiol. Scand 60:189

    PubMed  Google Scholar 

  • Helman, S.I., Fisher, R.S. 1976. Localization and determination of the driving force for sodium transport by the frog skin.Fed. Proc. 35:702

    Google Scholar 

  • Helman, S.I., Fisher, R.S. 1977. Microelectrode studies of the active Na transport pathway of frog skin.J. Gen. Physiol. (in press)

  • Koefoed-Johnson, V., Ussing, H.H. 1958. The nature of the frog skin potential.Acta Physiol. Scand. 42:298

    PubMed  Google Scholar 

  • Leblanc, G. 1972. The mechanism of lithium accumulation in the isolated frog skin epithelium.Pfluegers Arch. 337:1

    Article  Google Scholar 

  • Lindley, B.D., Hoshiko, T. 1964. The effects of alkali metal cations and common anions on the frog skin potential.J. Gen. Physiol. 47:749

    PubMed  Google Scholar 

  • Morel, F., Leblanc, G. 1975. Transient current changes and Na compartmentalization in frog skin epithelium.Pfluegers Arch. 358:135

    Article  Google Scholar 

  • Nagel, W. 1975. IntracellularPD of frog skin epithelium.Pfluegers Arch. 355:R70

    Google Scholar 

  • Nagel, W. 1976a. Na transport properties of the apical border of the frog skin epithelium.Physiologist 19:308

    Google Scholar 

  • Nagel, W. 1976b. The intracellular electrical potential profile of the frog skin epithelium.Pfluegers Arch. 365:135

    Article  Google Scholar 

  • Nagel, W. 1976c. Intercellular junctions of frog skin epithelial cells.Nature (London) 264:469

    Google Scholar 

  • Nagel, W. 1977a. Effect of high [K] upon the frog skin intracellular potentials.Pfluegers Arch. 368:R22

    Google Scholar 

  • Nagel, W. 1977b. The dependency of the electrical potential across the membranes of the frog skin epithelium upon the epithelial [Na].J. Physiol. (London) 269:777

    Google Scholar 

  • Nagel, W., Helman, S.I. 1977. Evidence for electrogenic transport of Na in frog skin revealed in microelectrode studies.Pfluegers Arch. 368:R22

    Google Scholar 

  • Reinach, P.S., Candia, O.A., Siegel, G.J. 1975. Lithium transport across isolated frog skin epithelium.J. Membrane Biol. 25:75

    Article  Google Scholar 

  • Rick, R., Dörge, A., Nagel, W. 1975. Influx and efflux of sodium at the outer surface of frog skin.J. Membrane Biol. 22:183

    Article  Google Scholar 

  • Teorell, T. 1954. Rhythmical potential and impedance variations in isolated frog skin induced by lithium ions.Acta Physiol. Scand. 31:268

    PubMed  Google Scholar 

  • Thellier, M., Lasalles, J.-P., Stelz, Th., Hartmann, A., Ayadi, A. 1976. Oscillations de potentiel et de courant électriques transépitheliaux sous l'effet du lithium.C. R. Acad. Sci. Ser. D. 282:2111

    Google Scholar 

  • Zerahn, K. 1955. Studies on the active transport of lithium in the isolated frog skin.Acta Physiol. Scand. 33:347

    PubMed  Google Scholar 

  • Zylber, E.A., Rotunno, C.A., Cereijido, M. 1973. Ion and water balance in isolated epithelial cells of the abdominal skin of the frog Leptodactylus ocellatus.J. Membrane Biol. 13:199

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology, University of Munich, Pettenkoferstr. 12, D-8000, Munich 2, Germany

    Wolfram Nagel

Authors
  1. Wolfram Nagel
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nagel, W. Influence of lithium upon the intracellular potential of frog skin epithelium. J. Membrain Biol. 37, 347–359 (1977). https://doi.org/10.1007/BF01940939

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01940939

Key words

Search

Navigation