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Transmural differences in myocardial blood flow and in coronary dilatory capacity in hemodiluted conscious dogs

Transmurale Unterschiede der Myokarddurchblutung und der Koronarreserve in wachen Hunden bei Hämodilution

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Summary

In 16 conscious resting dogs regional myocardial blood flow and the local coronary dilatory capacity were studied with the particle distribution technique during isovolemic hemodilution (hct=13%). Postischemic peak coronary hyperemia following release of temporary circumflex coronary artery occlusion was used for quantification of regional coronary dilatory capacity. In hemodilution (arterial blood oxygen content less than one third of normal) left ventricular blood flow (LVBF) was 460±36 ml/100 g·min, subendocardial/subepicardial flow amounted to 1.3±0.1. During postischemic peak hyperemia LVBF increased by 33% up to 606±63 ml/100 g·min. This 33% increase in LVBF was distributed mainly to the subepicardial layer, while in the subendocardial layer there was no significant flow increase. It is concluded that the increase in heart rate and systolic coronary vascular compression in addition to the lowered arterial oxygen content lead to exhaustion of the dilatory reserve in the subendocardium during hemodilution. Therefore the remainingoverall dilatory capacity is without functional significance.

Zusammenfassung

Die Verteilung der Myokarddurchblutung und der Dilatationsreserve der Koronargefäße wurde in 16 wachen Hunden in Ruhelage mit der Partikelverteilungsmethode bestimmt bei isovolämischer Hämodilution. Die postischämische Hyperämie der Koronargefäße nach Eröffnung eines zeitweiligen Verschlusses der Zirkumflexarterie wurde zur Beurteilung der lokalen Dilatationsreserve herangezogen. Unter Hämodilution (mit einem arteriellen Sauerstoffgehalt von weniger als einem Drittel des Normalwertes) betrug die Durchblutung des linken Ventrikels 460±36 ml/100 g·min.Das Verhältnis von subendokardialer/subepikardialer Durchblutung betrug 1,3±0,1. Während postischämischer Hyperämie stieg die Ventrikeldurchblutung um 33% auf 606±63 ml/100 g·min. Diese 33% ige Durchblutungszunahme erstreckte sich hauptsächlich auf die subepikardiale Schicht der Ventrikelwand, während in der subendokardialen Schicht keine signifikante Durchblutungszunahme nachweisbar war. Es wird gefolgert, daß die Zunahme der Herzfrequenz und der systolischen Koronargefäßkompression zusammen mit dem erniedrigten arteriellen Sauerstoffgehalt zu dieser Erschöpfung der Dilatationsreserve in der Innenschicht unter Hämodilution führen. Deshalb ist die verbleibende gemittelte Dilatationsreserve der gesamten Ventrikelwand ohne funktionelle Bedeutung.

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References

  1. Messmer, K., H. Schmid-Schönbein (Eds.), Hemodilution. Theoretical basis and Clinical Application (Basel 1972).

  2. Restorff, W. v., B. Höfling, J. Holtz, E. Bassenge, Effect of improved fluidity through hemodilution on coronary circulation at rest and during exercise. Pflügers Arch.357, 15–24 (1975).

    Google Scholar 

  3. Restorff, W. v., B. Höfling, J. Holtz, E. Bassenge Effect of improved fluidity through hemodilution on general circulation at rest and during exercise. Pflügers Arch.357, 25–34 (1975).

    Google Scholar 

  4. Downey, J. M., E. S. Kirk, Distribution of the coronary blood flow across the canine heart wall during systole. Circulat. Res.34, 251–257 (1974).

    PubMed  Google Scholar 

  5. Downey, J. M., H. F. Downey, E. S. Kirk, Effects of myocardial strains on coronary blood flow. Circulat. Res.34, 286–292 (1974).

    PubMed  Google Scholar 

  6. Myers, W. W., C. R. Honig, Number and distribution of capillaries as determinants of myocardial oxygen tension. Amer. J. Physiol.207, 653–660 (1964).

    PubMed  Google Scholar 

  7. Buckberg, G. D., A. A. Kattus, Factors determining the distribution and adequacy of left ventricular myocardial blood flow. Adv. Exp. Med. Biol.39, 95–113 (1973).

    PubMed  Google Scholar 

  8. Khouri, E. M., D. E. Gregg, C. R. Rayford, Effect of exercise on cardiac output, left coronary flow and myocardial metabolism in the unanesthetized dog. Circulat. Res.17, 427–437 (1965).

    PubMed  Google Scholar 

  9. Finsterer, U., P. Prucksunand, H. Brechtelsbauer, Critical evaluation of methods for determination of blood volume in the dog. Pflügers Arch.341, 63–72 (1973).

    Google Scholar 

  10. Wüsten, B., W. Flameng, W. Schaper, The distribution of myocardial flow. Part I: Effects of experimental coronary occlusion. Basic Res. Cardiol.69, 422–434 (1974).

    PubMed  Google Scholar 

  11. Rudolph, A. M., M. A. Heymann, The circulation of the fetus utero. Methods for studying distribution of blood flow, cardiac output and organ flow. Circulat. Res.21, 163–184 (1967).

    PubMed  Google Scholar 

  12. Schaper, W., P. Lewi, W. Flameng, L. Gijpen, Myocardial steal produced by coronary vasodilation in chronic coronary artery occlusion. Basic Res. Cardiol.68, 3–20 (1973).

    PubMed  Google Scholar 

  13. Buckberg, G. D., J. C. Luck, D. B. Payne, J. P. Archie, D. E. Fixler, Some sources of error in measuring regional blood flow with radioactive microspheres. J. Appl. Physiol.31, 598–604 (1971).

    PubMed  Google Scholar 

  14. Domenech, R. J., J. I. E. Hoffman, M. I. M. Noble, K. B. Saunders, J. R. Henson, S. Subijunto, Total and regional coronary blood flow measured by radioactive microspheres in conscious and anesthetized dogs. Circulat. Res.25, 581–596 (1969).

    PubMed  Google Scholar 

  15. Sugishita, Y., S. Kaihara, H. Yasuda, M. Iio, S. Murao, H. Ueda, Myocardial distribution of blood flow in the dog studied by the labeled microsphere. Part I. Method. Jap. Heart J.12, 50–59 (1971).

    Google Scholar 

  16. Gregg, D. E., E. M. Khouri, C. R. Rayford, Systemic and coronary energetics in the resting unanesthetized dog. Circulat. Res.16, 102–113 (1965).

    PubMed  Google Scholar 

  17. Utley, J., E. L. Carlson, J. I. E. Hoffman, H. M. Martinez, G. D. Buckberg, Total and regional myocardial blood flow measurements with 25 μ, 15 μ, 9 μ, and filtered 1–10 μ diameter microspheres and antipyrine in dogs and sheep. Circulat. Res.34, 391–405 (1974).

    PubMed  Google Scholar 

  18. Yipintsoi, T., W. A. Dobbs, P. D. Scanlon, T. J. Knopp, J. B. Bassingthwaighte, Regional distribution of diffusible tracers and carbonized microspheres in the left ventricle of isolated dog hearts. Circulat. Res.33, 573–587 (1973).

    PubMed  Google Scholar 

  19. Reneman, R. S., A. H. M. Jageneau, W. v. Gerven, J. Dony, P. Beirnaert, The radioactive microsphere method for the assessment of regional myocardial blood flow after coronary artery occlusion. Pflügers Arch.353, 337–347 (1975).

    Google Scholar 

  20. Fixler, D. E., M. Wheeler, D. Huffines, Extent of myocardial flow from luminal collateral circulation. J. Appl. Physiol.37, 282–285 (1974).

    PubMed  Google Scholar 

  21. Breull, W., H. Dahners, D. Fink, D. Redel, W. K. Raff, F. W. Schulz, H. Flohr, Effect of increasing left ventricular enddiastolic pressure on the transmural distribution of myocardial blood flow. Pflügers Arch.355, R 26 (1975).

    Google Scholar 

  22. Kjekshus, J. K., Mechanism for flow distribution in normal and ischemic myocardium during increased ventricular preload in the dog. Circulat. Res.33, 489–499 (1973).

    PubMed  Google Scholar 

  23. Buckberg, G., J. Brazier, Coronary blood flow and cardiac function during hemodilution. Bibl. Hämatol.41, 152–162 (1975).

    Google Scholar 

  24. Schmid-Schönbein, H., R. E. Wells, Rheological properties of human erythrocytes and their influence upon the “anomalous” viscosity of blood. Ergebn. Physiol.63, 145–219 (1971).

    Google Scholar 

  25. Olsson, R. A., D. E. Gregg, Myocardial reactive hyperemia in the unanesthetized dog. Amer. J. Physiol.208, 224–230 (1965).

    PubMed  Google Scholar 

  26. Höfling, B., E. Bassenge, W. v. Restorff, J. Holtz, K. Overshol, Inertial pressure loss in coronary perfusion of the beating dog heart. Pflügers Arch.347, R 4 (1974).

    Google Scholar 

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Author notes

  1. J. Holtz

    Present address: Institute of Physiology, Ludwig Maximilian University of Munich, Pettenkoferstraße 12, 8000, Munich, Germany

Authors and Affiliations

  1. Institute of Physiology, Ludwig Maximilian University of Munich, Pettenkoferstraße 12, 8000, Munich, Germany

    E. Bassenge, W. von Restorff & E. Mayer

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  1. J. Holtz
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  2. E. Bassenge
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  3. W. von Restorff
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  4. E. Mayer
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Holtz, J., Bassenge, E., von Restorff, W. et al. Transmural differences in myocardial blood flow and in coronary dilatory capacity in hemodiluted conscious dogs. Basic Res Cardiol 71, 36–46 (1976). https://doi.org/10.1007/BF01907781

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  • DOI: https://doi.org/10.1007/BF01907781

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