Abstract
Following the application of high pressure to skeletal muscle for extended periods, intracristal structures are found in the mitochondria. In addition, electron dense granules of 70–170 nm diameter are found in the matrix of these mitochondria. In contrast, pressure-treated liver mitochondria show only large (300–400 nm) matrix granules but not the intracristal structures. Both the inner and outer mitochondrial membranes appear intact after pressure-treatment. Short periods of pressuretreatment have little effect on either the morphology of mitochondria or the pH of the tissues. It is suggested that the formation of the intracristal structures may be due to the effects of pH rather than pressure alone. This finding raises the possibility that intracristal structures may occur as a preparative artefact particularly where the tissue has undergone considerable manipulation.
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S. B. Zimmerman and A. M. Zimmerman, in:High Pressure Effects on Cellular Processes, A. M. Zimmerman (ed.), Academic Press, New York, 1970, p. 179.
J. D. Hall and F. L. Crane,Exp. Cell Res.,62 (1970) 480.
J. D. Hall and F. L. Crane,J. Cell Biol.,48 (1971) 420.
T. Wakabayashi, J. M. Smoly, O. Hatase and D. E. Green,J. Bioenergetics,2 (1971) 167.
J. G. Hirsch and M. E. Fedorko,J. Cell Biol.,38 (1968) 615.
E. S. Reynolds,J. Cell Biol.,17 (1963) 208.
J. B. Landau and L. Thibodeau,Exp. Cell Res.,27 (1962) 591.
H. J. Deuticke and U. Ebbecke,Hoppe-Seylers Z. für Physiol. Chem.,247 (1937) 79.
M. J. Legato, D. Spiro and G. A. Langer,J. Cell Biol.,37 (1968) 1.
L. D. Peachey,J. Cell Biol.,20 (1964) 95.
K. Porter,J. Cell Biol.,20 (1964) 95.
M. Klingenberg and E. Pfaff, in:Regulation of Metabolic Processes in Mitochondria, J. M. Tager, S. Papa, E. Quagliariello and E. C. Slater (eds.), Elsevier Publishing Co., Amsterdam, 1966, p. 180.
G. L. Sottocasa, G. Sandri, E. Panfili and B. DeBernard,FEBS Letters,17 (1971) 100.
T. Suzuki and F. K. Mostofi,J. Cell Biol.,33 (1967) 605.
E. Pihl and G. F. Bahr, Exp. Cell Res.,63 (1970) 391.
R. B. Jennings, P. B. Herdson and H. M. Sommers,Lab. Invest.,20 (1969) 548.
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Morton, D.J., Rowe, R.W.D. & Macfarlane, J.J. The formation of intracristal structures induced in skeletal muscle mitochondria by high pressure. J Bioenerg Biomembr 4, 445–453 (1973). https://doi.org/10.1007/BF01648971
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DOI: https://doi.org/10.1007/BF01648971