Summary
A number of 2-deoxy-d-glucose (2-DOG) resistant mutants exhibiting resistance to glucose repression were isolated from variousSaccharomyces yeast strains. Most of the mutants isolated were observed to have improved maltose uptake ability in the presence of glucose. Fermentation studies indicated that maltose was taken up at a faster rate and glucose taken up at a slower rate in the mutant strains compared to the parental strains, when these sugars were fermented together. When these sugars were fermented separately, only the 2-DOG resistant mutant obtained fromSaccharomyces cerevisiae strain 1190 exhibited alterations in glucose and maltose uptake compared to the parental strain. Kinetic analysis of sugar transport employing radiolabelled glucose and maltose indicated that both glucose and maltose were transported with higher rates in the mutant strain. These results suggested that the high affinity glucose transport system was regulated by glucose repression in the parental strain but was derepressed in the mutant.
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Allen, K.E., M.T. McNally, H.S. Lowendorf, C.W. Slayman and S.J. Free. 1989. Deoxyglucose-resistant mutants ofNeurospora crassa: Isolation, mapping and biochemical characterization. J. Bacteriol. 171: 53–58.
Bailey, R.B. and A. Woodward. 1984. Isolation and characterization of a pleiotropic glucose repression resistant mutant ofSaccharomyces cerevisiae. Mol. Gen. Genet. 193: 507–512.
Barnett, J.A., 1976. The utilization of sugars by yeasts. Adv. Carbohydr. Chem. Biochem. 32: 125–234.
Biely, P., Z. Kratky and S. Bauer. 1972. Metabolism of 2-deoxy-d-glucose by baker's yeast. IV. Incorporation of 2-deoxy-d-glucose into cell wall mannan. Biochim. Biophys. Acta. 255: 631–639.
Bisson, L.F. 1989. High-affinity glucose transport inSaccharomyces cerevisiae is under general glucose repression control. J. Bacteriol. 170: 4838–4845.
Bisson, L.F. and D.G. Fraenkel. 1983. Involvement of kinases in glucose and fructose uptake bySaccharomyces cerevisiae. Proc. Natl. Acad. Sci. U.S.A. 80: 1730–1734.
Bisson, L.F. and D.G. Fraenkel. 1984. Expression of kinase dependent glucose uptake inSaccharomyces cerevisiae. J. Bacteriol. 159: 1013–1017.
Busturia, A. and R. Lagunas. 1985. Identification of two forms of the maltose transport systems inSaccharomyces cerevisiae and their regulation by catabolite inactivation. Biochim. Biophys. Acta 820: 324–326.
Buttner, R., A. Scheit, R. Bode and D. Birnbaum. 1989. Isolation and characterization of mutants ofTrichosporon adeninovorans resistant to 2-deoxyglucose. J. Basic Microbiol. 29: 76–82.
Cabeca-Silva, C. 1982. Enhanced production of amylases by derepressed mutants of the yeastEndomycopsis fibuligera. Cienc. Biol. (Portugal) 7: 65–70.
Carlson, M. 1987. Regulation of sugar utilization inSaccharomyces species. J. Bacteriol. 169: 4873–4877.
D'Amore, T., I. Russell and G.G. Stewart. 1989. Sugar utilization by yeast during fermentation. J. Ind. Microbiol. 4: 315–324.
D'Amore, T., I. Russell and G.G. Stewart. 1989. The effect of carbohydrate adjuncts on brewer's wort fermentation bySaccharomyces uvarum (carlsbergensis). J. Inst. Brew. 95: 333–336.
Dhawale, M.R. and W.M. Ingledew. 1983. Starch hydrolysis of derepressed mutants ofSchwanniomyces castellii. Biotechnol. Lett. 5: 185–190.
Does, A.L. and L.F. Bisson. 1989. Comparison of glucose uptake kinetics in different yeasts. J. Bacteriol. 171: 1303–1308.
Erratt, J.A. and G.G. Stewart. 1981. Fermentation studies usingSaccharomyces diastaticus yeast strains. Develop. Ind. Microbiol. 22: 577–586.
Gancedo, J.M. and C. Gancedo. 1986. Catabolite repression mutants in yeast. FEMS Microbiol. Rev. 32: 179–187.
Gorts, C.P.M. 1969. Effect of glucose on the activity and the kinetics of the maltose-uptake system and of α-glucosidase inSaccharomyces cerevisiae. Biochim. Biophys. Acta 184: 299–305.
Heredia, C.F. and A. Sols. 1964. Metabolic studies with 2-deoxyhexoses. II. Resistance to 2-deoxyglucose in a yeast mutant. Biochim. Biophys. Acta 86: 224–228.
Heredia, M.F. and C.F. Heredia. 1988.Saccharomyces cerevisiae acquires resistance to 2-deoxyglucose at a very high frequency. J. Bacteriol. 170: 2870–2872.
Holzer, H. 1976. Catabolite inactivation in yeast. Trends Biochem. Sci. 1: 178–180.
Jones, R.M., I. Russell and G.G. Stewart. 1986. The use of catabolite derepression as a means of improving the fermentation rate of brewing yeast strains. J. Am. Soc. Brew. Chem. 44: 161–166.
Lobo, Z. and P.K. Maitra. 1977. Resistance to 2-deoxyglucose in yeast. Direct selection of mutants lacking glucose-phosphorylating enzymes. Mol. Gen. Genet. 157: 297–300.
Maitra, P.K. and Z. Lobo. 1981. Genetics of glucose phosphorylation in yeast. In: Current Developments In Yeast Research (Stewart, G.G. and I. Russell, eds.), pp. 293–297, Pergamon Press, Toronto.
Russell, I. 1988. Studies on yeast with improved carbohydrate utilization. Ph.D. Dissertation, University of Strathclyde, Glasgow, Scotland.
Seaston, A., C. Inkson and A.A. Eddy. 1973. The absorption of protons with specific amino acids and carbohydrates by yeast. Biochem. J. 134: 1031–1043.
Serrano, R. 1977. Energy requirements for maltose transport in yeast. Eur. J. Biochem. 80: 97–102.
van Uden, N., C. Cabeca-Silva, A. Madeira-Lopes and I. Spencer-Martins. 1980. Selective isolation of derepressed mutants of an α-amylase yeast by the use of 2-deoxyglucose. Biotechnol. Bioeng. 22: 651–654.
Zimmermann, F.K. and I. Scheel. 1977. Mutants ofSaccharomyces cerevisiae resistant to carbon catabolite repression. Mol. Gen. Genet. 154: 75–82.
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Novak, S., D'Amore, T., Russell, I. et al. Characterization of sugar transport in 2-deoxy-d-glucose resistant mutants of yeast. Journal of Industrial Microbiology 6, 149–155 (1990). https://doi.org/10.1007/BF01576435
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DOI: https://doi.org/10.1007/BF01576435