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Sugar transport systems in Kluyveromyces marxianus CCT 7735

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Abstract

The pattern of glucose repression in most Kluyveromyces marxianus strains does not correlate with fermentative behaviour; however, glucose repression and fermentative metabolism appear to be linked to the kinetics of sugar uptake. In this work, we show that lactose transport in K. marxianus CCT 7735 by lactose-grown cells is mediated by a low-affinity H+-sugar symporter. This system is glucose repressed and able to transport galactose with low affinity. We also observed the activity of a distinct lactose transporter in response to raffinose. Regarding glucose uptake, specificities of at least three low-affinity systems rely on the carbon source available in a given growth medium. Interestingly, it was observed only one high-affinity system is able to transport both glucose and galactose. We also showed that K. marxianus CCT 7735 regulates the expression of sugar transport systems in response to glucose availability.

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Acknowledgements

This study was supported by the Brazilian Agencies CNPq (National Science and Technology Development Council), CAPES (Coordination for the Improvement of Higher Education Personnel) and FAPEMIG (Foundation for Research Support of the State of Minas Gerais). We also would like to acknowledge Federal University of Ouro Preto, MG, Brazil for financial support (Process N. 23109.003209/2016-98 Edital PROPP 09/2016) and Federal University of Viçosa, MG, Brazil.

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Authors and Affiliations

  1. Departamento de Microbiologia Agrícola, BIOAGRO, Universidade Federal de Viçosa, Av. PH Rolfs s/n, 36571-000, Viçosa, MG, Brazil

    Fernando Augusto da Silveira, Raphael Hermano S. Diniz & Wendel B. da Silveira

  2. Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus do Morro do Cruzeiro - 35.400-000, Ouro Preto, MG, Brazil

    Raphael Hermano S. Diniz, Geraldo M. S. Sampaio, Rogelio L. Brandão & Ieso M. Castro

  3. Instituto Federal de Educação, Ciência e Tecnologia de Minas Gerais - Campus Ouro Preto, CODACIB, Ouro Preto, MG, Brazil

    Raphael Hermano S. Diniz

Authors
  1. Fernando Augusto da Silveira
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  2. Raphael Hermano S. Diniz
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  3. Geraldo M. S. Sampaio
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  4. Rogelio L. Brandão
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  5. Wendel B. da Silveira
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  6. Ieso M. Castro
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Contributions

Considering the involvement, explained below, we believe that it is appropriate to include the following authors in the manuscript: Silveira FA, Diniz RHS, Sampaio GSS, Brandao, RL, Silveira WB and Castro IM. IMC and WBS: Conceived of or designed study. FAS, RHSD and GMSS: Performed research. IMC, WBS and RLB: Analyzed data and discussion relative. IMC and WBS: Wrote the paper.

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Correspondence to Ieso M. Castro.

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This work has not been published previously, it is not under consideration for publication anywhere else, and its publication in Antonie van Leeuwenhoek has been approved by all co-authors, as well as by the responsible authorities at the institutes where the work has been carried out. The authors ensure that accepted principles of ethical and professional conduct has been followed and absence of conflicts of interest.

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da Silveira, F.A., Diniz, R.H.S., Sampaio, G.M.S. et al. Sugar transport systems in Kluyveromyces marxianus CCT 7735. Antonie van Leeuwenhoek 112, 211–223 (2019). https://doi.org/10.1007/s10482-018-1143-4

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