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A Growth-Based Screening System for Hexose Transporters in Yeast

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Glucose Transport

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1713))

Abstract

As the simplest eukaryotic model system, the unicellular yeast Saccharomyces cerevisiae is ideally suited for quick and simple functional studies as well as for high-throughput screening. We generated a strain deficient for all endogenous hexose transporters, which has been successfully used to clone, characterize, and engineer carbohydrate transporters from different source organisms. Here we present basic protocols for handling this strain and characterizing sugar transporters heterologously expressed in it.

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Acknowledgement

We thank Dr. Joanna Tripp for helpful comments on the manuscript.

Author information

Authors and Affiliations

  1. Institute of Molecular Biosciences, Goethe University, Biozentrum, Frankfurt am Main, Max-von-Laue Straße 9, Biozentrum, 60438, Frankfurt, Germany

    Eckhard Boles & Mislav Oreb

Authors
  1. Eckhard Boles
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  2. Mislav Oreb
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Corresponding author

Correspondence to Eckhard Boles .

Editor information

Editors and Affiliations

  1. Department of Experimental Medical Science, Lund University, Lund, Sweden

    Karin Lindkvist-Petersson

  2. Department of Experimental Medical Science, Lund University, Lund, Sweden

    Jesper S Hansen

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Boles, E., Oreb, M. (2018). A Growth-Based Screening System for Hexose Transporters in Yeast. In: Lindkvist-Petersson, K., Hansen, J. (eds) Glucose Transport. Methods in Molecular Biology, vol 1713. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7507-5_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7507-5_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7506-8

  • Online ISBN: 978-1-4939-7507-5

  • eBook Packages: Springer Protocols

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