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Pollen tube extract supports the movement of actin filaments in vitro

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Summary

Muscle actin filaments labeled with rhodamine-phalloidin were observed to move on the surface coated with a crude extract of pollen tubes ofLilium longiflorum with an average velocity of 1.99±0.55 μm/sec. The movement required both Mg2+ and ATP. These results indicate that the extract of pollen tubes contains a myosin-like translocator

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Abbreviations

ATP:

adenosine-5′-triphosphate

DTT:

dithiothreitol

EGTA:

ethyleneglycol-bis-(β-aminoethylether)N,N,N′,N′-tetraacetic acid

PIPES:

piperazine-N,N′-bis-(2-ethanesulfonic acid)

PMSF:

phenylmethylsulfonyl fluoride

References

  • Collins K, Sellers JR, Matsudaira P (1990) Calmodulin dissociation regulates brush border myosin I (110-kD-calmodulin) mechanochemical activity in vitro. J Cell Biol 110: 1137–1147

    Google Scholar 

  • Harada Y, Noguchi A, Kishino A, Yanagida T (1987) Sliding movement of single actin filaments on one-headed myosin filaments. Nature 326: 805–808

    Google Scholar 

  • Heslop-Harrison J (1987) Pollen germination and pollen-tube growth. Int Rev Cytol 107: 1–78

    Google Scholar 

  • Kohama K (1981) Amino acid incorporation rates into myofibrillar proteins of dystrophic chicken skeletal muscle. J Biochem 90: 497–501

    Google Scholar 

  • Kohno T, Shimmen T (1988 a) Accelerated sliding of pollen tube organelles alongCharaceae actin bundles regulated by Ca2+. J Cell Biol 106: 1539–1543

    Google Scholar 

  • — — (1988 b) Mechanism of Ca2+ inhibition of cytoplasmic streaming in lily pollen tubes. J Cell Sci 91: 501–509

    Google Scholar 

  • —, Chaen S, Shimmen T (1990) Characterization of the transloeator associated with pollen tube organelles. Protoplasma 154: 179–183

    Google Scholar 

  • Kron SJ, Spudich JA (1986) Fluorescent actin filaments move on myosin fixed to a glass surface. Proc Natl Acad Sci USA 83: 6272–6276

    Google Scholar 

  • Okagaki T, Higashi-Fujime S, Kohama K (1989) Ca2+ activates actin-filament sliding on scallop myosin but inhibits that onPhysarum myosin. J Biochem 106: 955–957

    Google Scholar 

  • Steer MW, Steer JM (1989) Pollen tube tip growth. New Phytol 111: 323–358

    Google Scholar 

  • Takiguchi K, Hayashi H, Kurimoto E, Higashi-Fujime S (1990) In vitro motility of skeletal muscle myosin and its proteolytic fragments. J Biochem 107: 671–679

    Google Scholar 

  • Yano-Toyoshima Y, Krön SJ, McNally EM, Niebling KR, Toyoshima C, Spudich JA (1987) Myosin subfragment-1 is sufficient to move actin filaments in vitro. Nature 328: 536–539

    Google Scholar 

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Authors and Affiliations

  1. Department of Botany, Faculty of Science, University of Tokyo, Hongo, Tokyo

    T. Kohno

  2. The Physical Science Laboratories, Nishon University at Narashino, Funabashi, Chiba

    T. Okagaki

  3. Department of Pharmacology, Gunma University, School of Medicine, Maebashi, Gunma

    K. Kohama

  4. Department of Life Science, Faculty of Science, Himeji Institute of Technology, 2167 Shosha, Himeji, 671-22, Hyogo, Japan

    T. Shimmen

Authors
  1. T. Kohno
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  2. T. Okagaki
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  3. K. Kohama
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  4. T. Shimmen
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Kohno, T., Okagaki, T., Kohama, K. et al. Pollen tube extract supports the movement of actin filaments in vitro. Protoplasma 161, 75–77 (1991). https://doi.org/10.1007/BF01328900

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  • DOI: https://doi.org/10.1007/BF01328900

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