Abstract
The actin cytoskeleton plays an essential role in the regulation of polarized pollen tube growth, and its functions are dictated by its spatial organization and dynamics. Here we describe an assay to monitor the dynamics of actin filaments decorated with Lifeact-mEGFP in Arabidopsis pollen tubes using spinning disk confocal microscopy and measuring the parameters associated with their dynamics. The method allows us to assess the dynamics of actin filaments in growing Arabidopsis pollen tubes.
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Acknowledgments
This work was supported by grants from National Natural Science Foundation of China (32270338 and 31970180) and the startup fund from Huazhong Agricultural University. We thank Dr. Wanying Zhao for the help in drawing the schematic diagram shown in Fig. 4.
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Lu, Q., Liu, X., Qu, X., Huang, S. (2023). Visualization and Quantification of the Dynamics of Actin Filaments in Arabidopsis Pollen Tubes. In: Hussey, P.J., Wang, P. (eds) The Plant Cytoskeleton. Methods in Molecular Biology, vol 2604. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2867-6_23
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DOI: https://doi.org/10.1007/978-1-0716-2867-6_23
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Online ISBN: 978-1-0716-2867-6
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