Summary
Mouse neuroblastoma (MNB) cells infected with ERA virus were specifically lysed in the presence of rabbit complement by antisera produced in mice to challenge virus standard (CVS), ERA, Flury HEP or street virus (SV) strains of rabies. MNB or EL-4 cells persistently infected with ERA, MNB cells infected with CVS, and BHK-21/S13 cells infected with ERA or Flury HEP also were suitable targets. CER cells infected with either ERA, CVS or Flury HEP, BHK-21/S13 cells infected with CVS and MNB cells infected with Flury HEP were not suitable targets. Two unusual findings indicated that 1. some cells which were >80 percent positive for rabies viral membrane antigens(s) were poorly lysed, and 2. some cells that expressed cytoplasmic antigen lacked detectable membrane antigens.
Similar content being viewed by others
References
Arai, Y. T., Kondo, A., Suzuki, K.: Demonstration of non-infectious hemagglutinating particles of rabies virus and isolation of the hemagglutinin by disruption of the virion with nonidet P-40. Arch. Virol.51, 335–345 (1976).
Bell, J. F.: Abortive rabies infection. I. Experimental production in white mice and general discussions. J. inf. Dis.114, 249–257 (1964).
Bell, J. F., Lodmell, D. L., Moore, G. J., Raymond, G. H.: Brain neutralization of rabies virus to distinguish recovered animals from previously vaccinated animals. J. Immunol.97, 747–753 (1966).
Chesebro, B., Wehrly, K., Chesebro, K., Portis, J.: Characterization of Ia 8 antigen, thy-1.2 antigen, complement receptors, and virus production in a group of murine virus-induced leukemia cell lines. J. Immunol.117, 1267–1274 (1976).
Fernandes, M. V., Wiktor, T. J., Koprowski, H.: Endosymbiotic relationship between animal viruses and host cells: A study of rabies virus in tissue culture. J. exp. Med.120, 1099–1116 (1964).
Hummeler, K., Koprowksi, H., Wiktor, T. J.: Structure and development of rabies virus in tissue culture. J. Virol.1, 152–170 (1967).
Iwasaki, Y., Clark, H. F.: Rabies virus infection in mouse neuroblastoma cells. Lab. Invest.36, 578–584 (1977).
Iwasaki, Y., Wiktor, T. J., Koprowski, H.: Early events of rabies virus replication in tissue cultures. An electron microscopic study. Lab. Invest.28, 142–148 (1973).
Koprowski, H.: Immunopathology of rabies virus infection. Symp. Series Immunobiol. Standard.21, 89–101 (1974).
Lodmell, D. L., Bell, J. F., Moore, G. J., Raymond, G. H.: Comparative study of abortive and nonabortive rabies in mice. J. inf. Dis.119, 569–580 (1969).
MacPherson, I., Stoker, M.: Polyoma transformation of hamster cell clones — an investigation of genetic factors affecting cell competence. Virology16, 147–151 (1962).
Smith, A. L., Tignor, G. H., Mifune, K., Motohashi, T.: Isolation and assay of rabies serogroup viruses in CER cells. Intervirology8, 92–99 (1977).
Wiktor, T. J., Clark, H. F.: Chronic rabies virus infection of cell cultures. Inf. Immun.6, 988–995 (1972).
Wiktor, T. J., Koprowski, H.: Monoclonal antibodies against rabies virus produced by somatic cell hybridization: Detection of antigenic variants. Proc. Natl. Acad. Sci. U.S.A.75, 3938–3942 (1978).
Wiktor, T. J., Kuwert, E., Koprowski, H.: Immune lysis of rabies virus-infected cells. J. Immunol.101, 1271–1282 (1968).
Author information
Authors and Affiliations
Additional information
With 2 Figures
Rights and permissions
About this article
Cite this article
Lodmell, D.L., Arai, Y.T. & Ewalt, L.C. Influence of cell type and virus upon lysis of rabies virus-infected cells by antibody and complement. Archives of Virology 70, 147–155 (1981). https://doi.org/10.1007/BF01315008
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01315008