Summary
In this paper we present further studies on the generation of tissue cultures from leaves of the cerealSorghum bicolor (L.) Moench. It could be shown that during differentiation the leaf tissue rapidly loses the ability to respond to conventional tissue culture techniques. This was probably related to a loss of sensitivity towards 2,4-D, an otherwise most potent growth regulator in tissue culture. The immature tissue which proved to be sensitive proliferated over a wide range of concentration with a broad optimum of about 0.6–6 mg 1−1 2,4-D. This concentration range appears to be only slightly higher than that described for many dicotyledonous tissue cultures. The relevance of these findings is discussed with reference to the well known dual function of 2,4-D, namely as a selective herbicide and a potent artificial auxin. The implications of these attributes to the practical application of cereal tissue culture is stressed.
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Abbreviations
- 2,4-D:
-
2,4-Dichlorophenoxyacetic acid
- 4-CPA:
-
4-Chlorophenoxyacetic acid
- NAA:
-
1-Naphthaleneacetic acid
- IAA:
-
3-Indoleacetic acid
- Kinetin:
-
6-Furfurylaminopurine
- 6-BAP:
-
6-Benzylaminopurine
- GA3 :
-
Gibberellic acid
- ABA:
-
Abscisic acid
- MS:
-
Murashige and Skoog
References
Begg, J. E., Wright, M. J., 1962: Growth and development of leaves from intercalary meristems inPhalaris arundinacea L. Nature194, 1097–1098.
Brettell, R., Wernicke, W., Thomas, E., 1980: Embryogenesis from cultured immature inflorescences ofSorghum bicolor. Protoplasma104, 141–148.
Chen, H.-M., Teng, S.-Y., Yu, J.-J., 1980: Callus induction and organ formation from young leaf of wheat (Triticum aestivum). Acta Botanica Sinica22, 112–116.
Chu, C.-C., Wang, C.-C., Sun, C.-S., Hsü, C., Yin, K.-C., Chu, C.-Y., Bi, F.-Y., 1975: Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scientia Sinica18, 659–668.
Deka, P. C., Sen, S. K., 1976: Differentiation in calli originated from isolated protoplasts of rice (Oryza sativa L.) through plating technique. Molec. gen. Genet.145, 239–243.
Esau, K., 1977: Anatomy of Seed Plants. New York: J. Wiley and Sons Inc.
Gamborg, O. L., Miller, R. A., Ojima, K., 1968: Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res.50, 151–158.
Geyer, G., 1973: Ultrahistochemie. Stuttgart: G. Fischer.
Haydu, Z., Vasil, I. K., 1981: Somatic embryogenesis and plant regeneration from leaf tissue and anthers ofPennisetum Schum. Theor. appl. Genet.59, 269–273.
Heinz, D. J., Mee, G. W. P., 1969: Plant differentiation from callus tissue ofSaccharum species. Crop Sci.9, 346–348.
Kefeli, V. J., 1978: Natural Plant Growth Inhibitors and Phytohormones. The Hague: Dr. W. Junk Publishers.
King, P. J., Potrykus, I., Thomas, E., 1978: In vitro genetics of cereals: problems and perspectives. Physiol. Vég.16, 381–399.
Klämbt, H. D., 1961: Wachtumsinduktion und Wuchsstoffmetabolismus im Weizenkoleoptilzylinder, Teil III. Planta57, 339–353.
Lo, P. F., Chen, C. H., Ross, J. G., 1980: Vegetative propagation of temperate forage grasses through calles culture. Crop Sci.20, 363–367.
Lu, C., Vasil, I. K., 1981: Somatic embryogenesis and plant regeneration from leaf tissue ofPanicum maximum Jacq. Theor. appl. Genet.59, 275–280.
Murashige, T., Skoog, F., 1962: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant.15, 473–497.
Nickell, L. G., 1977: Crop improvement in sugarcane: studies using in vitro methods. Crop Sci.17, 717–719.
Nitsch, J. P., 1969: Experimental androgenesis inNicotiana. Phytomorphology19, 389–404.
O'Hara, J. F., Street, H. E., 1978: Wheat callus culture: the initiation, growth and organogenesis of callus derived from various explant sources. Ann. Bot.42, 1029–1038.
Sekiya, J., Yasuda, T., Yamada, Y., 1977: Callus induction in tobacco, pea, rice and barley plants by auxins and their analogues. Plant Cell Physiol.18, 1155–1157.
Sharman, B. C., 1941: Development of the ligule inZea mays L. Nature147, 641.
—, 1942: Developmental anatomy of the shoot ofZea mays L. Ann. Bot.6, 245–282.
Spurr, A. R., 1969: A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res.26, 31–43.
Street, H. E., 1977: Plant Tissue and Cell Culture. Oxford: Blackwell Scientific Publ.
Thomas, E.,Wernicke, W., 1978: Morphogenesis in herbaceous crop plants. In: Frontiers of Plant Tissue Culture (Thorpe, A., ed.), pp. 403–410. Calgary.
—,Brettell, R., Potrykus, I., Wernicke, W., 1980: Cereal tissue cultures. In: Tissue Culture Methods for Plant Pathologists (Ingram, D., Helgeson, J., eds.), pp. 41–49. Oxford: Blackwell Scientific Publ.
Vasil, V., Vasil, I. K., 1980: Isolation and culture of cereal protoplasts: 2. Embryogenesis and plantlet formation from protoplasts ofPennisetum americanum. Theor. appl. Genet.56, 97–99.
Wernicke, W., Brettell, R., 1980: Somatic embryogenesis fromSorghum bicolor leaves. Nature287, 138–139.
—,Potrykus, I., Thomas, E., 1982: Morphogenesis from cultured leaf tissue ofSorghum bicolor —The morphogenetic pathways. Protoplasma111, 53–62.
—,Brettell, R., Wakizuka, T., Potrykus, I., 1981: Adventitious embryoid and root formation from rice leaves. Z. Pflanzenphysiol.103, 361–366.
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Wernicke, W., Brettell, R.I.S. Morphogenesis from cultured leaf tissue ofSorghum bicolor-culture initiation. Protoplasma 111, 19–27 (1982). https://doi.org/10.1007/BF01287643
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DOI: https://doi.org/10.1007/BF01287643