Abstract
This protocol describes in vitro plant regeneration from mature female inflorescence explants of date palm (Phoenix dactylifera L.) by reversion of floral state (reproductive phase) to the vegetative state. The mature female inflorescence (fully developed) is cultured on MS induction medium containing 10 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), 3 mg/L 2-isopentenyladenine (2iP), and 2 mg/L paclobutrazol (PBZ) or 2 mg/L abscisic acid (ABA). The basal part of the petals has meristematic cells, which can be induced to initiate callus or direct shoot formation depending on the plant growth regulator amendments. Callus forms on the induction medium supplemented with PBZ after 12 weeks, whereas it differentiates into somatic embryos on a medium containing 0.1 mg/L naphthaleneacetic acid (NAA). Direct shoots are regenerated on the induction medium amended with ABA after 24 weeks. Procedures for plant regeneration from mature female inflorescence explants are described, and histological changes which occur during the reversion process are presented.
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References
Loutfi K, Chlyah H (1998) Vegetative multiplication of date palms from in vitro cultured inflorescences: effect of some growth regulator combinations and organogenetic potential of various cultivars. Agronomie 18:573–580
Abahman L (2010) Date palm (Phoenix dactylifera L.) micropropagation from inflorescence tissues by using somatic embryogenesis technique. Acta Hortic 882:827–832
Abul-Soad AA (2011) Micropropagation of date palm using inflorescence explants. In: Jain SM, Al-Khayri JM, Johnson DV (eds) Date palm biotechnology. Springer, Dordrecht, pp 91–118
Zayed EMM (2011) Propagation of Phoenix dactylifera L. Chamaerops humilis L. and Hyophorbe verschaffeltii L. palms by using tissue culture technique. Ph.D. Thesis, Department of Horticulture, Faculty of Agriculture Cairo University, Egypt, p 167
Kriaa W, Sghaier B, Masmoudi F, Benjemaa R, Drira N (2012) The date palm (Phoenix dactylifera L.) micropropagation using completely mature female flowers. C R Biol 335:194–204
Zayed EMM, Abdelbar OH (2015) Morphogenesis of immature female inflorescences of date palm in vitro. Ann Agric Sci 60(1):113–120
Zayed EMM, Zein El-Din AFM, Manaf HH, Abdelbar OH (2016) Floral reversion of mature inflorescences of date palm in vitro. Ann Agric Sci 61(1):125–133
De Mason D, Stolte W, Tisserat B (1982) Floral development in Phoenix dactylifera. Can J Bot 60(8):1437–1446
George EF, Hall MA, De Klerk GJ (eds) (2008) Plant growth regulators III. In: Plant propagation by tissue culture. Springer, Dordrecht, pp 227–283
Rademacher W (1991) Biochemical effects of plant growth retardants. In: Gausman HW (ed) Plant biochemical regulators. Marcel Dekker Inc., New York, pp 169–200
Marshall JG, Rutledge RG, Blumwald E, Dumbroff EB (2000) Reduction in turgid water volume in jack pine, white spruce and black spruce in response to drought and paclobutrazol. Tree Physiol 20:701–707
Suboti A, Jevremovi S, Trifunovi M, Petri M, Miloševi S, Grubiši D (2009) The influence of gibberellic acid and paclobutrazol on induction of somatic embryogenesis in wild type and hairy root cultures of Centaurium erythraea Gillib. Afr J Biotech 8(14):3223–3228
Sethi U, Basu A, Mukherjee SG (1990) Role of inhibitors in the induction of differentiation in callus cultures of Brassica, Datura and Nicotiana. Plant Cell Rep 8:598–600
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Mater AA (1986) In vitro propagation of Phoenix dactylifera L. Date Palm J 4(2):137–152
Omar MS (1988) In vitro response of various date palm explants. Date Palm J 6(2):371–388
Johansen DA (1940) Plant microtechnique. McGraw-Hill Book Co., New York, pp 126–156
Huala E, Sussex IM (1993) Determination and cell interaction in reproductive meristems. Plant Cell 5:1157–1165
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Zayed, E.M.M. (2017). Direct Organogenesis and Indirect Somatic Embryogenesis by In Vitro Reversion of Mature Female Floral Buds to a Vegetative State. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume I. Methods in Molecular Biology, vol 1637. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7156-5_5
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DOI: https://doi.org/10.1007/978-1-4939-7156-5_5
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Online ISBN: 978-1-4939-7156-5
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