Summary
Phosphoenolpyruvate carboxylase (E. C. 4.1.1.31) was localized in the guard cells by an indirect, immunofluorescent technique using antisera raised against the enzyme from bothPennisetum glaucum R. Br. (pearl millet) andEscherichia coli. Of the 17 species of plants examined, only monocot species showed fluorescence with millet phosphoenolpyruvate carboxylase antiserum and onlyNephrolepis exaltata (L.) Schott,Zygocactus truncatus Haworth, andEcheveria gibbiflora D. C. displayed fluorescence withE. coli phosphoenolpyruvate carboxylase antiserum.
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Madhavan, S., Smith, B.N. Phosphoenolpyruvate carboxylase in guard cells of several species as determined by an indirect, immunofluorescent technique. Protoplasma 122, 157–161 (1984). https://doi.org/10.1007/BF01281692
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DOI: https://doi.org/10.1007/BF01281692