Abstract
Specific labeling of proteins and nucleic acids by immunofluorescence or in situ techniques is an important adjunct to microscopical analysis for cell biology. Labeling of nuclear structures in intact complex tissues is often hampered by problems of penetration of the macromolecular labeling reagents needed. Here we describe a method of labeling isolated plant nuclei that we have found to be a useful approach that can help to overcome these problems.
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Acknowledgments
This work was supported by grant BB/J004588/1 from BBSRC and the John Innes Foundation. We thank Drs Azahara MartÃn and Stefanie Rosa for helpful comments on the manuscript.
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Pendle, A., Shaw, P. (2016). Immunolabeling and In Situ Labeling of Isolated Plant Interphase Nuclei. In: Kianian, S., Kianian, P. (eds) Plant Cytogenetics. Methods in Molecular Biology, vol 1429. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3622-9_6
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DOI: https://doi.org/10.1007/978-1-4939-3622-9_6
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3620-5
Online ISBN: 978-1-4939-3622-9
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