Summary
Using indirect immunofluorescence on both dissociated cell cultures and frozen sections from rat sciatic nerve, dorsal root ganglion and superior cervical ganglion, we have examined the development and distribution of the peripheral myelin protein P2. It first appears in development in sciatic nerve and dorsal root ganglion on the first day after birth, at about the same time as P0 and P1. Like galactocerebroside, P0 and P1, P2 disappears gradually from dissociated Schwann cells in culture. In adult sciatic nerve, and dorsal and ventral roots, it shows an uneven distribution and is absent from some myelinated axons. In electron micrographs the onset of myelination in the sciatic nerve occurs between the day of birth (day 0) and the first day after birth (day 1). Immunofluorescence studies on freshly dissociated cell suspensions, frozen sections and dissociated cell cultures at these early time points indicate that the myelin glycolipids galactocerebroside and sulphatide are present on the surface of many Schwann cells at least one day before myelination starts while the myelin proteins P0, P1 and P2 are not detected until myelination begins. This suggests that the early appearance of galactocerebroside and sulphatide is an important step preceding the formation of compact myelin.
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Brockes, J. P., Fields, K. L. &Raff, M. C. (1979) Studies on cultured rat Schwann cells. I. Establishment of purified populations from cultures of peripheral nerve.Brain Research 165, 105–18.
Brockes, J. P., Raff, M. C., Nishiguchi, D. J. &Winter, J. (1980) Studies on cultured rat Schwann cells, III. Assays for peripheral myelin proteins.Journal of Neurocytology 9, 67–77.
Brostoff, S. W. &Eylar, E. H. (1972) The proposed amino acid sequence of P1 protein of rabbit sciatic nerve myelin.Archives of Biochemistry and Biophysics 153, 590–8.
Brostoff, S. W., Sacks, H., Dalcanto, M., Johnson, A. B., Raine, C. S. &Wisniewski, H. (1974) The P2 protein of bovine root myelin: isolation and some chemical and immunological properties.Journal of Neurochemistry 23, 1037–43.
Eylar, E. H., Szymanska, I., Ishaque, A., Ramwani, J. &Dubiski, S. (1980) Localization of the P2 protein in peripheral nerve myelin.Journal of Immunology 124, 1086–92.
Fields, K. L., Brockes, J. P., Mirsky, R. &Wendon, L. M. B. (1978) Cell surface markers for distinguishing different types of rat dorsal root ganglion cells in culture.Cell 14, 43–51.
Fields, K. L., Gosling, C., Megson, M. &Stern, P. L. (1975) New cell surface antigens in rat defined by tumours of the nervous system.Proceedings of the National Academy of Sciences USA 72, 1286–300.
Greenfield, S., Brostoff, S. W. &Hogan, E. L. (1980) Characterization of the basic proteins from rodent peripheral nervous system myelin.Journal of Neurochemistry 34, 453–5.
Greenfield, S. W., Eylar, E. H. &Morell, P. (1973) Protein composition of myelin of the peripheral nervous system.Journal of Neurochemistry 20, 1207–16.
Kadlubowski, M. &Hughes, R. A. C. (1980) Neurogenicity and encephalitogenicity of P2 in rat, guinea pig and rabbit.Journal of the Neurological Sciences 48, 171–8.
Kadlubowski, M., Hughes, R. A. C. &Gregson, N. A. (1980) Experimental allergic neuritis in the Lewis rat: characterization of the activity of peripheral myelin and its major basic protein P2.Brain Research 184, 439–54.
Kitamura, K., Suzuki, M., Suzuki, A. &Uremura, K. (1980) The complete amino acid sequence of the P2 protein in bovine peripheral nerve myelin.FEBS Letters 115, 27–30.
Mirsky, R., Winter, J., Abney, E. R., Pruss, R. M., Gavrilovic, J. &Raff, M. C. (1980) Myelin-specific proteins and glycolipids in rat Schwann cells and oligodendrocytes in culture.Journal of Cell Biology 84, 483–94.
Trapp, B. D., Mcintrye, L. J., Quarles, R. H., Sternberger, N. H. &Webster, H. DeF. (1979) Immunocytochemical localization of rat peripheral nervous system myelin proteins: P2 protein is not a component of all peripheral nervous system myelin sheaths.Proceedings of the National Academy of Sciences USA 76, 3552–6.
Wood, J. G. &Engel, E. L. (1976) Peripheral nerve glycoproteins and myelin fine structure during development of rat sciatic nerve.Journal of Neurocytology 5, 605–15.
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Winter, J., Mirsky, R. & Kádlubowski, M. Immunocytochemical study of the appearance of P2 in developing rat peripheral nerve: comparison with other myelin components. J Neurocytol 11, 351–362 (1982). https://doi.org/10.1007/BF01257982
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DOI: https://doi.org/10.1007/BF01257982