Abstract
Treatment of cultured pancreatic B-cells (HIT-T15 and RINm5F) with the diabetogenic drug Streptozotocin resulted in a significant increase in the number of cells that became detached from the substrate during a subsequent culture period. Examination of the detached cells by fluorescence microscopy after staining with acridine orange or by electron microscopy revealed evidence of chromatin condensation and margination. Isolation and fractionation of DNA from these cells revealed a pattern of oligonucleosomal fragmentation that was not evident in untreated cells. All of these features are characteristic of entry of the cells into apoptosis and the results suggest that the diabetogenic action of Streptozotocin involves induction of apoptosis in pancreatic B-cells.
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References
Rakieten, N., Rakieten, M. L. and Nadkarni, M. V. (1963)Cancer Chemo. Rep. 29:91–98.
Okamoto, H. (1992) In:Nutrient regulation of insulin secretion (Flatt, P. R. ed.) Portland Press, UK, pp. 387–406.
Okamoto, H. (1990) In:Molecular Biology of the Islets of Langerhans (Okamoto, H. ed.) Cambridge University Press, UK, pp. 209–231.
Schein, P. S., Cooney, D. A., McMenamin, M. G. and Anderson, T. (1973)Biochem. Pharmacol. 22:2625–2631.
Hinz, M., Katsilambros, N., Maier, V., Schatz, H. and Pfeiffer, E. F. (1973) FEBS Lett.30:225–228.
Eizirik, D. L., Sandler, S., Ahnstrom, G. and Welsh, M. (1991)Biochem. Pharmacol. 42:2275–2282.
Kawada, J. (1992)Yakugaku Zasshi 112:773–791.
Yamamoto, H., Uchigata, Y. and Okamoto, H. (1981)Nature 294:284–286.
Okamoto, H. (1981)Mol. Cell. Biochem. 37:43–61.
Turk, J., Corbett, J. A., Ramanadham, S., Bohrer, A. and McDaniel, M. L. (1993)Biochem. Biophys. Res. Comm. 197:1458–1464.
O'Neill, S. K., Dutta, S. and Triggle, C. R. (1993)Proc. Western Pharmacol. Soc. 36:203–207.
LeDoux, S. P., Woodley, S. E., Patton, N. J. and Wilson, G. L. (1986)Diabetes 35:866–872.
Hayaishi, O. and Ueda, K. (1977)Ann. Rev. Biochem. 46:95–116.
Wyllie, A. H., Kerr, J. F. R. and Currie, A. R. (1980)Int. Rev. Cytol. 68:251–306.
Wyllie, A. H. (1980)Nature 284:555–556.
Williams, G. T., Smith, C. A., McCarthy, N. J. and Grimes, E. A. (1992)Trends Cell. Biol. 2:263–267.
Williams, G. T. and Smith, C. A. (1993)Cell 74:777–779.
Dive, C. and Hickman, J. A. (1991)Br. J. Cancer 64:192–196.
Catchpole, D. R. and Stewart, B. W. (1993)Cancer Res. 53:4287–4296.
Fisher, T. C.et al. (1993)Cancer Res. 53:3321–3326.
Smith, C. A., Williams, G. T., Kingston, R., Jenkinson, E. J. and Owen, J. J. T. (1989)Nature 337:181–184.
Rasschaert, J., Eizirik, D. L. and Malaisse, W. J. (1992)Endocrinology 130:3522–3528.
Sofue, M., Yoshimura, Y., Nishida, M. and Kawada, J. (1991)J. Endorinol. 131:135–138.
Yamamoto, H. and Okamoto, H. (1982)FEBS Lett. 145:298–302.
Southern, C., Schulster, D. and Green, I. C. (1990)FEBS Lett. 276:42–44.
Corbett, J. A.et al. (1992)Biochem. J. 287:229–235.
Delaney, C. A., Green, M. H. L., Lowe, J. E. and Green, I. C. (1993)FEBS Lett. 333:291–295.
Tuch, B. E. and Chen, J. (1993)Pancreas 8:305–311.
Moreira, J. E.et al. (1991)Virchows Archiv 60:337–344.
Juntti-Berggren, L.et al. (1993)Science 261:86–90.
Lorenzo, A., Razzaboni, B., Weir, G. C. and Yanker, B. A. (1994)Nature 368:756–760.
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Morgan, N.G., Cable, H.C., Newcombe, N.R. et al. Treatment of cultured pancreatic B-cells with streptozotocin induces cell death by apoptosis. Biosci Rep 14, 243–250 (1994). https://doi.org/10.1007/BF01209729
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DOI: https://doi.org/10.1007/BF01209729