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A simple method for large-scale electrofusion and culture of plant protoplasts

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Bioscience Reports

Abstract

A simple method is described for the electrofusion of plant protoplasts. Protoplasts were aggregated in a radio-frequency field (10 V RMS, 0.5 MHz) for 15-30 s with an inter-electrode distance of J mm. They were then fused with a 300-V DC pulse. The protoplasts were able to divide after this treatment. A trans-ferrable electrode permitted electrofusion of l-ml volumes of culture in standard tissue-culture dishes in about 20 s.

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Authors and Affiliations

  1. John Innes Institute, Colney Lane, NR4 7UH, Norwich, UK

    J. W. Watts & Janet M. King

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  1. J. W. Watts
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  2. Janet M. King
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Watts, J.W., King, J.M. A simple method for large-scale electrofusion and culture of plant protoplasts. Biosci Rep 4, 335–342 (1984). https://doi.org/10.1007/BF01140497

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  • DOI: https://doi.org/10.1007/BF01140497

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