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Microinjection of cultured cells using red-cell-mediated fusion and osmotic lysis of pinosomes: A review of methods and applications

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Abstract

Proteins and other macromolecules can be injected into cultured cells by several different methods. Here we review the strengths and limitations of two of these methods, red-cell-mediated microinjection and osmotic: lysis of pinosomes, and indicate how they may be successfully applied to the study of cultured cells.

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References

  1. Stacey DW & Allfrey VG (1977) J. Cell Biol.75, 807–817.

    PubMed  Google Scholar 

  2. Graessmann A, Graessmann M & Mueller C (1979) Biochem. Biophys. Res. Commun.88, 428–432.

    PubMed  Google Scholar 

  3. Kulka RG & Loyter A (1979) in: Current Topics in Membranes and Transport, vol 12 (Bronner F & Kleinzeller A, eds), pp 366–430, Academic Press, Inc.

  4. Furusawa M, Nishimura T, Yamaizumi M & Okada Y (1974) Nature249, 449–450.

    PubMed  Google Scholar 

  5. Schlegel RA & Rechsteiner MC (1975) Cell5, 371–379.

    PubMed  Google Scholar 

  6. Loyter A, Zakai N & Kulka RG (1975) J. Cell Biol.66, 292–304.

    PubMed  Google Scholar 

  7. Kimelberg HK & Mayhew E (1978) CRC Crit. Rev. Toxicol.6, 25–79.

    Google Scholar 

  8. Pagano RE & Weinstein JN (1978) Annu. Rev. Biophys. Bioeng.7, 435–468.

    PubMed  Google Scholar 

  9. Okada CY & Rechsteiner M (1982) Cell29, 33–41.

    PubMed  Google Scholar 

  10. Diacumakos EG (1980) in: Introduction of Macromolecules into Viable Mammalian Cells, vol 1 (Baserga R, Croce C & Rovera G, eds), pp 85–98, Alan R Liss, Inc, New York.

    Google Scholar 

  11. Diacumakos EG, Holland S, & Pecora P (1970) Proc. Natl. Acad. Sci. USA65, 911–919.

    PubMed  Google Scholar 

  12. Taber R, Tazewell W & Papahadjopoulos D (1980) in: Introduction of Macromolecules into Viable Mammalian Cells, vol 1 (Baserga R, Croce C & Rovera G, eds), pp 221–238, Alan R Liss, Inc, New York.

    Google Scholar 

  13. Szoka F, Jacobson K, Derzko Z & Papahajopoulos D (1980) Biochim. Biophys. Acta600, 1–18.

    PubMed  Google Scholar 

  14. Neff NT, Bourret L, Miao P & Dice JF (1981) J. Cell Biol.91, 184–194.

    PubMed  Google Scholar 

  15. Dice JF (1982) J. Biol. Chem.257, 14624–14627.

    PubMed  Google Scholar 

  16. Backer JM, Bourret L & Dice JF (1983) Proc. Natl. Acad. Sci. USA80, 2166–2170.

    PubMed  Google Scholar 

  17. McElligott MA & Dice JF (1983) Biochem. J.216 559–566.

    PubMed  Google Scholar 

  18. Ihler G, Glew RH & Schnure FW (1973) Proc. Natl. Acad. Sci.70, 2663–2666.

    PubMed  Google Scholar 

  19. Rechsteiner MC (1975) Exptl. Cell Res.93, 487–492.

    PubMed  Google Scholar 

  20. Schlegel RA & Rechsteiner MC (1978) Meth. Cell Biol.20, 341–354.

    Google Scholar 

  21. Seeman P (1967) J. Cell Biol.32, 55–70.

    PubMed  Google Scholar 

  22. Kriegler MP & Livingston DM (1977) Somatic Cell Genetics3, 603–610.

    PubMed  Google Scholar 

  23. Kaltoft K, Zeuthen J, Engbaek F, Piper PW & Celis JE (1976) Proc. Natl. Acad. Sci. USA73, 2793–2797.

    PubMed  Google Scholar 

  24. Asano K, Murachi T & Asano A (1983) J. Biochem.93, 733–741.

    PubMed  Google Scholar 

  25. Kruse CA, Spector EB, Cederbaum SD, Wisnieski BJ & Popjak G (1981) Biochim. Biophys. Acta645, 339–345.

    PubMed  Google Scholar 

  26. Vainstein A, Razin A, Graessman A & Loyter A (1983) Meth. Enzymol. 101, 492–512.

    PubMed  Google Scholar 

  27. Beigel M & Loyter A (1983) Exp. Cell Res.148, 95–103.

    PubMed  Google Scholar 

  28. Wojcieszyn JW, Schlegel RA, Lumley-Sapanski K & Jacobson KA (1983) J. Cell Biol.96, 151–159.

    PubMed  Google Scholar 

  29. Boni LT, Stewart TP, Alderfer JL & Hui SW (1981) J. Memb. Biol.62, 65–70.

    Google Scholar 

  30. Maggio B, Ahkong QF & Lucy JA (1976) Biochem. J.158, 647–650.

    PubMed  Google Scholar 

  31. Knutton S (1979) J. Cell Sci.36, 61–72.

    PubMed  Google Scholar 

  32. Honda K, Maeda Y, Sasakawa S, Ohno H & Tsuchida E (1981) Biochem. Biophys. Res. Commun.101, 165–171.

    PubMed  Google Scholar 

  33. Smith CL, Ahkong QF, Fisher D & Lucy J (1982) Biochim. Biophys. Acta692, 109–114.

    PubMed  Google Scholar 

  34. Bigelow S, Hough R & Rechsteiner M (1981) Cell25, 83–93.

    PubMed  Google Scholar 

  35. Dice JF, Bourret L & Miao P (1981) J. Cell Biol.91, 241a.

    Google Scholar 

  36. McGarry T, Hough R, Rogers S & Rechsteiner M (1983) J. Cell Biol.96, 338–346.

    PubMed  Google Scholar 

  37. Rechsteiner M, Chin D, Hough R, McGarry T, Rogers S, Rote K & Wu L (1984) in: Cell Fusion, Ciba Foundation Symposium103, pp 181–195, Pitman, London.

    Google Scholar 

  38. Hendil KB (1980) J. Cell Physiol.105, 449–460.

    PubMed  Google Scholar 

  39. Zavortink M, Thacher T & Rechsteiner M (1979) J. Cell Physiol.100, 175–186.

    PubMed  Google Scholar 

  40. Wasserman M, Kulka RG & Loyter A (1977) FEBS Lett.83, 48–52.

    PubMed  Google Scholar 

  41. Mayer RJ, Evans P, Russell S & Amenta JS (1984) in: Cell Fusion, Ciba Foundation Symposium103, pp 202–214, Pitman, London.

    Google Scholar 

  42. Rechsteiner M & Kuehl L (1979) Cell16, 901–908.

    PubMed  Google Scholar 

  43. Atidia J & Kulka RG (1982) FEBS Lett.142, 72–76.

    PubMed  Google Scholar 

  44. Chin DT, Kuehl L & Rechsteiner M (1982) Proc. Natl. Acad. Sci.79, 5857–5861.

    PubMed  Google Scholar 

  45. Katznelson R & Kulka RG (1983) J. Biol. Chem.258, 9597–9600.

    PubMed  Google Scholar 

  46. Yamaizumi M, Uchida T, Mekada E & Okada Y (1979) Cell18, 1009–1014.

    PubMed  Google Scholar 

  47. Rote KV & Rechsteiner M (1983) J. Cell Physiol.116, 103–111.

    PubMed  Google Scholar 

  48. Hendil KB (1981) Exp. Cell Res.135, 157–166.

    PubMed  Google Scholar 

  49. Freikopf-Cassel A & Kulka RG (1981) FEBS Lett.128, 63–66.

    PubMed  Google Scholar 

  50. Wojcieszyn JW, Schlegel RA, Wu ES & Jacobson KA (1981) Proc. Natl. Acad. Sci. USA78, 4407–4410.

    PubMed  Google Scholar 

  51. Bennett FC, Busch H, Lischwe MA & Yeoman LC (1983) J. Cell Biol.97, 1556–1572.

    Google Scholar 

  52. Wu L, Rechsteiner M & Kuehl L (1981) J. Cell Biol.91, 488–496.

    PubMed  Google Scholar 

  53. Boogaard C & Dixon GH (1983) Exp. Cell Res.143, 191–205.

    PubMed  Google Scholar 

  54. Boogaard C & Dixon GH (1983) Exp. Cell Res.143, 175–190.

    PubMed  Google Scholar 

  55. Kaltoft K & Celis JE (1978) Exp. Cell. Res.115, 423–428.

    PubMed  Google Scholar 

  56. Antman KH & Livingston DM (1980) Cell19, 627–635.

    PubMed  Google Scholar 

  57. Yamaizumi M, Uchida T, Okada Y & Furusawa M (1978) Cell13, 227–232.

    PubMed  Google Scholar 

  58. Boney C, Fink D, Schlichter D, Carr K & Wicks WD (1983) J. Biol. Chem.258, 4911–4918.

    PubMed  Google Scholar 

  59. Ohara J, Sugi M, Fujimoto M & Watanabe T (1982) J. Immunol.129, 1227–1232.

    PubMed  Google Scholar 

  60. Ohara J & Watanabe T (1982) J. Immunol.128, 1090–1096.

    PubMed  Google Scholar 

  61. Kriegler MP, Griffen JD & Livingston DM (1978) Cell14, 983–994.

    PubMed  Google Scholar 

  62. Straus SE & Raskas HJ (1980) J. Gen. Virol.48, 241–245.

    PubMed  Google Scholar 

  63. Mercer WE, Terefinko DI & Schlegel RA (1979) Cell Biol. Intl. Repts.3, 265–270.

    Google Scholar 

  64. Kriegler MP & Livingston DM (1980) in: Introduction of Macromolecules into Viable Mammalian Cells, vol 1 (Baserga R, Croce C & Rovera G, eds) pp 261–296, Alan R Liss, Inc, New York.

    Google Scholar 

  65. Kishimoto T, Miki Y, Kishi H, Muraguchi A, Kishimoto S & Yamamura Y (1982) J. Immunol.129, 1367–1371.

    PubMed  Google Scholar 

  66. Morrison M & Schonbaum GR (1976) Ann. Rev. Biochem.45, 861–888.

    PubMed  Google Scholar 

  67. Kim S & Paik WK (1965) J. Biol. Chem.240, 4629–4634.

    PubMed  Google Scholar 

  68. Tweto J & Doyle D (1976) J. Biol. Chem.251, 872–882.

    PubMed  Google Scholar 

  69. Evans PJ & Mayer RJ (1982) Biochem. Biophys. Res. Commun.107, 51–58.

    PubMed  Google Scholar 

  70. Dice JF, Hess EJ & Goldberg AL (1979) Biochem. J.178, 305–312.

    PubMed  Google Scholar 

  71. Furusawa M (1980) Int. Rev. Cytol.62, 29–67.

    PubMed  Google Scholar 

  72. Docherty PA & Aronson NN (1982) Biochem. Biophys. Res. Commun.109, 527–532.

    PubMed  Google Scholar 

  73. Wasserman M, Zakai N, Loyter A & Kulka RG (1976) Cell7, 551–556.

    PubMed  Google Scholar 

  74. Smith JD & Liu AY-C (1981) Sci. 214, 799–800.

    Google Scholar 

  75. Godfrey W, Doe B & Wofsy L (1983) Proc. Natl. Acad. Sci. USA80, 2267–2271.

    PubMed  Google Scholar 

  76. Brown DB, Hanks SK & Rao PN (1981) J. Cell Biol.91, 12a.

    Google Scholar 

  77. Zimmerman U & Vienken J (1982) J. Memb. Biol.67, 165–182.

    Google Scholar 

  78. Steinman RM, Mellman IS, Muller WA & Cohn ZA (1983) J. Cell Biol.96, 1–27.

    PubMed  Google Scholar 

  79. Merion M & Sly WS (1983) J. Cell Biol.96, 644–650.

    PubMed  Google Scholar 

  80. Storrie B, Pool RR Jr, Sachdera M, Maurey KM & Oliver C (1984) J. Cell. Biol.98, 108–115.

    PubMed  Google Scholar 

  81. FitzGerald DJP, Padmanabhan R, Pastan I & Willingham MC (1983) Cell32, 607–617.

    PubMed  Google Scholar 

  82. Johnson RT & Rao PN (1970) Nature226, 717–722.

    PubMed  Google Scholar 

  83. Croy RG & Pardee AB (1983) Proc. Natl. Acad. Sci.80, 4699–4703.

    PubMed  Google Scholar 

  84. Smith JH, Subbarao MN & Eliceiri GL (1983) J. Cell. Phys.114, 1–6.

    Google Scholar 

  85. Drescher-Lincoln CK & Smith JR (1983) Expt. Cell Res.144, 455–462.

    Google Scholar 

  86. Larner J, Cheng K, Schwartz C, Kikuchi K, Tamura S, Creacy S, Dubler R, Galasko G, Pullin C & Katz M (1982) Fed. Proc.41, 2724–2729.

    PubMed  Google Scholar 

  87. Seals JR & Czech MP (1982) Fed. Proc.41, 2730–2735.

    PubMed  Google Scholar 

  88. Schatz G & Butow RA (1983) Cell32, 316–318.

    PubMed  Google Scholar 

  89. Sabitini DD, Kreibich G, Morimoto T & Adesnik M (1982) J. Cell Biol.92, 1–22.

    PubMed  Google Scholar 

  90. Levinson AD, Courtneidge SA & Bishop JM (1981) Proc. Natl. Acad. Sci.78, 1624–1628.

    PubMed  Google Scholar 

  91. Tepperman J (1980) in: Metabolic and Endocrine Physiology, Fourth Edition, pp 21–28, Year Book Medical Publishers, Inc., Chicago, London.

    Google Scholar 

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  1. Department of Physiology and Biophysics, Harvard Medical School, 02115, Boston, MA, USA

    Mary Ann McElligott & J. Fred Dice

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  1. Mary Ann McElligott
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  2. J. Fred Dice
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McElligott, M.A., Dice, J.F. Microinjection of cultured cells using red-cell-mediated fusion and osmotic lysis of pinosomes: A review of methods and applications. Biosci Rep 4, 451–466 (1984). https://doi.org/10.1007/BF01122221

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