Abstract
The effect of therapeutic-intensity ultrasound on neuromuscular transmission and spontaneous electrical and contractile activity in smooth muscles of the gastrointestinal tract of guinea pig was studied by a modified sucrose-gap technique. The action of ultrasound was found to facilitate the acetylcholinergic neuromuscular transmission (mainly by increasing the amplitude of excitatory postsynaptic potentials). The higher efficiency of the nonadrenergic neuromuscular transmission was manifested as an increase (nearly twofold) in the total duration, but not in the amplitude, of inhibitory postsynaptic potentials. Modulations of the first and second components of the potentials caused respectively by the action of ATP and of nitric oxide as possible transmitters, were different. Concurrently with enhancing the synaptic transmission efficiency, ultrasound exerted an opposite, inhibitory, effect on generation of spontaneous action potentials and contraction of smooth muscles. All the ultrasound effects were fully reversible. The findings permit assuming a special mechanism of modification of the synaptic transmission in smooth muscles under the action of ultrasound.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
S. I. Zakharov, K. Yu. Bogdanov, L. R. Gavrilov, et al., “Ultrasound effect on contraction force and action potentials of papillary cardiac muscle of rat,”Byull. Éksp. Biol. Med.,107, No. 4, 423–426 (1989).
P. O. Makarov and A. V. Lonsky, “Ultrasound effect on nerve and single nerve fiber,”Biofizika,10, No. 1, 181–184 (1965).
V. I. Dreval', I. I. Zalyubovsky, N. D. Nazarenko, et al., “Ultrasound effect on structure of microsomal membranes,”Dokl. AN UkrSSR,5, No. 12, 51–54 (1985).
V. A. Selivanov, V. P. Zinchenko, and A. P. Sarvazyan, “On mechanism of low-intensity ultrasound action on mitochondrions,”Biofizika,27, No. 5, 653–656 (1982).
I. E. El'piner,Biophysics of Ultrasound [in Russian], Nauka, Moscow (1973).
J. M. Chapman, “The effect of ultrasound on the potassium content of rat thymocytesin vitro,”Brit. J. Radiol.,47, No. 3, 411–415 (1975).
G. L. Forester, A. J. Mortimer, D. Bateson, and W. J. Keon, “Ultrasonically induced changes in mouse skeletal musclein vivo,”Pflügers Arch.,400, No. 2, 208–210 (1984).
A. J. Mortimer and M. Dyson, “The effect of therapeutic ultrasound on calcium uptake in fibroblasts,”Ultrasound Med. Biol.,14, No. 3, 499–506 (1988).
E. B. Burlakova and N. G. Khrapova, “Changes in structural organization of lipids under the effect of ultrasound,”Usp. Khimii,54, No. 9, 1540–1542 (1985).
P. Carmona, M. Cozar, L. M. Garcia-Segula, and J. Monreal, “Conformation of brain proteolipid apoprotein. Effect of sonication and n-octyl-D-glucopyranosible detergent,”Eur. Biophys.,16, No. 3, 169–176 (1988).
W. Harvey, M. Dyson, J. B. Pond, and R. Grachame, “Thein vitro stimulation of protein synthesis in human fibroblast by therapeutic levels of ultrasound,” in:Ultrasonics in Medicine, E. Kazner et al. (eds.), Exepta Medica, Amsterdam (1975), pp. 10–21.
V. A. Buts and K. P. Skibenko, “Changes in immunogenicity of cells and supernatant under the effect of ultrasound,”Biofizika,36, No. 5, 863–865 (1991).
I. E. El'piner and L. M. Bronskaya, “The effect of ultrasonic waves on ATPases of plasma membranes,”Biofizika,15, No. 6, 852–856 (1970).
E. P. Chetverikova, T. N. Pashovkin, N. A. Rosanova, et al., “Interaction of therapeutic ultrasound with purifiedin vitro,”Ultrasonic, 23, No. 4, 183–188 (1985).
D. G. Talbert, “Spontaneous smooth muscle activity as a means of detecting biological effects of ultrasound,”Proc. Ultrasonics Inter., Guilford, 279–284 (1975).
I. V. Ostrovsky and I. V. Chizhmakov, “Ultrasonic effect on the membrane of an isolated neuron intensifies lidokain blocking of inward sodium current,”Dokl. AN SSSR,319, No. 4, 1003–1007 (1991).
V. A. Dyatlov, “Ultrasound effect on functional state of nicotinic acetylcholinoreceptors of molluscan neurons,”Neirofiziologiya,21, No. 5, 698–700 (1989).
D. P. Artemenko, V. A. Buryi, I. A. Vladimirova, and M. F. Shuba, “Modification of the sucrose-gap technique,”Fiziol. Zh.,28, No. 3, 374–380 (1982).
R. A. R. Bywater, M. E. Holman, and G. S. Taylor, “Atropine-resistance depolarization in the guine-pig intestine,”J. Physiol.,316, Jul., 47–56 (1981).
G. Burnstock, G. Campbell, D. G. Satchell, and A. Smythe, “Evidence that adenosine triphosphate or a related nucleotide is the transmitter substance released by non-adrenergic inhibitory nerves in the gut,”Brit. J. Pharmacol.,40, No. 5, 668–688 (1970).
I. A. Vladimirova and M. F. Shuba, “Effect of strychnine, hydrastatine, and apamin on synaptic transmission in smooth muscle cells,”Neirofiziologiya,10, No. 3, 295–299 (1978).
I. A. Vladimirova and M. F. Shuba, “Synaptic processes in smooth muscle,”Neirofiziologiya,16, No. 3, 307–319 (1984).
M. F. Shuba and I. A. Vladimirova, “Effect of apamin on the electrical response of smooth muscle to adenosine-5′-triphosphate and to non-adrenergic, non-cholinergic nerve stimulation,”Neuroscience,5, No. 5, 853–859 (1989).
V. P. Zagorodnyuk, I. A. Vladimirova, E. V. Vovk, and M. F. Shuba, “Studies of the inhibitory non-adrenergic neuro-muscular transmission in the smooth muscle of the normal human intestine and from case of Hirschrung's disease,”J. Auton. Nerv. Syst.,26, No. 1, 51–60 (1989).
Author information
Authors and Affiliations
Additional information
Neirofiziologiya/Neurophysiology, Vol. 25, No. 4, pp. 297–302, July–August, 1993.
Rights and permissions
About this article
Cite this article
Vladimirova, I.A., Zagorodnyuk, V.P., Ostrovsky, I.V. et al. Ultrasound-induced changes in synaptic processes with different transmitters in smooth muscles. Neurophysiology 25, 251–255 (1993). https://doi.org/10.1007/BF01054512
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01054512