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Electron transport-linked proton translocation at nitrite reduction inCampylobacter sputorum subspeciesbubulus

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Abstract

Campylobacter sputorum subspeciesbubulus contains a membrane-bound nitrite reductase which catalyses the six-electron reduction of nitrite to ammonia. Formate andL-lactate are used as hydrogen donors. Cells ofC. sputorum grown with nitrate or nitrite contain cytochromes of theb-andc-type and a carbon monoxide-binding cytochromec. In addition, a special membrane-bound carbon monoxide-binding pigment is found. Nitrite reduction with formate orL-lactate as a hydrogen donor is strongly inhibited by 2-n-heptyl-4-hydroxyquinoline-N-oxide (HQNO). Nitrite reduction by bacterial suspensions with lactate as a hydrogen donor is strongly inhibited by carbonylcyanide-m-chlorophenyl-hydrazone (CCCP) whereas nitrite reduction with formate as a hydrogen donor is not inhibited at all. →H+/O values and →H+/NO -2 values were measured with ascorbate + N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD), formate (in the absence and presence of carbonic anhydrase) andL-lactate as a hydrogen donor. The results are summarized in a scheme for electron transport from formate or lactate to oxygen or nitrite which shows a periplasmic orientation of formate dehydrogenase and nitrite reductase and a cytoplasmic orientation of lactate dehydrogenase and oxygen reduction, and which shows proton translocation with a →H+/2e value of 2.0. The →H+/O and →H+/NO -2 values predicted by this scheme are in good agreement with the experimental values.

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Abbreviations

CCCP:

carbonylcyanide-m-chlorophenylhydrazone

HQNO:

2-n-heptyl-4-hydroxyquinoline-N-oxide

MTPP+ :

methyltriphenylphosphonium cation

TMPD:

N,N,N′,N′-tetramethyl-p-phenylenediamine; →H+/O (→H+/NO -2 ), number of protons liberated in the outer bulk phase at the reduction of one atom O (one ion NO -2 ); →H+/2e (→q+/2e), number of protons (charges) translocated across the cytoplasmic membrane during flow of two electrons to an acceptor

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Author notes

  1. H. G. D. Niekus

    Present address: Department of Genetics, Delft, The Netherlands

Authors and Affiliations

  1. Biological Laboratory, Free University, De Boelelaan 1087, 1007 MC, Amsterdam, The Netherlands

    Wytske de Vries, H. G. D. Niekus, Hugerien van Berchum & A. H. Stouthamer

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  1. Wytske de Vries
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  2. H. G. D. Niekus
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  3. Hugerien van Berchum
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  4. A. H. Stouthamer
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de Vries, W., Niekus, H.G.D., van Berchum, H. et al. Electron transport-linked proton translocation at nitrite reduction inCampylobacter sputorum subspeciesbubulus . Arch. Microbiol. 131, 132–139 (1982). https://doi.org/10.1007/BF01053995

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  • DOI: https://doi.org/10.1007/BF01053995

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