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Effect of oxygen on antibody productivity in hybridoma culture

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Summary

The effect of the inlet gas oxygen concentration on hybridoma growth, viability and monoclonal antibody production was studied. Maximum viable cell cencentrations of 1.2×106 cells/mL were obtained in split-flow bioreactors mixed with a gas containing 10% oxygen. However, maximum antibody concentration was attained at a sub-optimal level of growth with an oxygen concentration of 2.5%.

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Authors and Affiliations

  1. Departments of Chemical Engineering and Biology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada

    H. A. Phillips, J. M. Scharer, N. C. Bols & M. Moo-Young

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  1. H. A. Phillips
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  2. J. M. Scharer
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  3. N. C. Bols
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  4. M. Moo-Young
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Phillips, H.A., Scharer, J.M., Bols, N.C. et al. Effect of oxygen on antibody productivity in hybridoma culture. Biotechnol Lett 9, 745–750 (1987). https://doi.org/10.1007/BF01028277

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  • DOI: https://doi.org/10.1007/BF01028277

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