Abstract
Adherent recombinant BHK cells were cultivated at temperatures between 30 and 37°C. Batch and repeated-batch-cultivations in a 2-litre bioreactor showed a significant influence on metabolism and cell growth. The low-temperature-cultivations showed a lower growth rate and a lower glucose consumption rate and, therefore, less lactate production. On the other hand, the maximum cell density and productivity seemed not to be affected by the temperature reduction.
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Weidemann, R., Ludwig, A. & Kretzmer, G. Low temperature cultivation — A step towards process optimisation. Cytotechnology 15, 111–116 (1994). https://doi.org/10.1007/BF00762385
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DOI: https://doi.org/10.1007/BF00762385