Abstract
The addition of ethanol extracts ofCentella asiatica showed a remarkable enhancement of fibroblast cells attachment to Cytodex beads in serum-free (SF) medium. It also improves tPA production in both batch and perfusion cultivations. The optimal concentration for SF medium was determined as 2 ppm of the extracts when using Cytodex III. In batch cultivation a high specific tPA production rate was obtained, compared to that from 5% FBS containing medium. However, a fast specific growth rate was observed in 5% FBS medium. In perfusion cultivation a reasonably good cell density and tPA production was achieved at a perfusion rate of 2.4×106 (viable cell/ml) and 0.65 (μg/ml), respectively at 22 ml/min.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahern TJ, Morris GE, Barne KM, Horgan PG, Timony GA, Angus LB, Henson KS, Stoudemire KB, Langer PR and Larsen GR (1990) Site-directed mutagenesis in human tissue plasminogen activator. J. Biol. Chem. 265: 5540–5545.
Buntemeyer H, Lutkemeyer D and Leheman J (1991) Optimization of serum-free fermentation processes for antibody production. Cytotechnology 5: 57–67.
Chasseaud LF, Fry BJ, Hawkins DR and Hathway DE (1971) The metabolism of asiatic acid, madecassic acid and asiaticoside in the rat. Drug Res. 9: 1379–1384.
Iscove NN and Melchers FJ (1978) Complete replacement of serum by albumin, transferrin and soybean lipid in cultures of B lymphocytes. J. Exp. Med. 147: 923–931.
Kodouri A and Bohak Z (1983) Productions of plasminogen activator in cultures of human fibroblasts. Bio/Technology 1: 354–358.
Karting T (1938) Clinical applications ofCentella asiatica (L.) In: Cracker LE and Simon JE (eds) Herbs, Spices and Medical Plants, Vol. 3 (pp. 145–173), Oryx Press, Arizona.
Lang AB, Schuerch U and Cryz SJ (1991) Optimization of growth and secretion of human monoclonal antibodies by hybridomas cultured in (SF) media. Hybridoma 10: 401–409.
Nam YK, Yu HK, Jeon BC, Deckwer WD and Lee HY (1993) Kinetic analysis of cellular nucleotides correlated to growth and tPA production in perfusion cultures of human fibroblast cells. Cytotechnology 11: 143–148.
Samoilovich SR, Dugan CB and Macario AJL (1987) Hybridoma technology: new development of practical interest. J. Immunol. Methods 101: 153–162.
Singh B and Rastogi RP (1969) A reinvestigation of the triterpenes ofCentella asiatica. Phytochem. 917–921.
Tao TY, Bohn MA, Ji G-Y, Einsele A and Hu WS (1987) Kinetics of prourokinase production by human kidney cells in culture. J. Biotechnol. 6: 205–224.
Vecchio DA, Senni I, Cossu G and Molinaro M (1984) Effects ofCentella asiatica on biosynthetic activity in cultured fubroblasts. Farmaco. Ed. Prat. 39: 355–364.
Vogel HG, Souza NJ and D'Sa A (1990) Effect of terpenoids isolated fromCentella asiatica on granuloma tissue. Acta Therapeutica 16: 285–298.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kim, Y.N., Park, Y.S., Kim, H.K. et al. Enhancement of the attachment on microcarriers and tPA production by fibroblast cells in a serum-free medium by the addition of the extracts ofCentella asiatica . Cytotechnology 13, 221–226 (1993). https://doi.org/10.1007/BF00749818
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00749818