Summary
We describe a method of measuring intestinal solute uptake that combines the virtues of simplicity, good tissue viability as reflected in high uptake rates, and reduction of unstirred layers comparable to the best reported for chambers. An everted sleeve of intestine is mounted on a grooved rod stationed immediately over a stirring bar rotating at 1,200 rpm. The effect of edge damage is negligible. The coefficient of variation of uptake measurements is 7%. For mouse intestine andd-glucose at high concentrations an incubation time of 1 min represents a suitable compromise among several competing criteria.l-glucose is used to correct actived-glucose uptake simultaneously for adherent fluid and for passive uptake. The technique's general utility is illustrated by its application to intestines of four species representing four classes of vertebrates.
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Karasov, W.H., Diamond, J.M. A simple method for measuring intestinal solute uptake in vitro. J Comp Physiol B 152, 105–116 (1983). https://doi.org/10.1007/BF00689734
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DOI: https://doi.org/10.1007/BF00689734