Summary
Rat brain was obtained on the 4th day after its damage by a stab wound. The injured and the normal control brain tissues were stained by the immunofluorescence technique using anti-granulomonocytic rabbit serum. After the fluorescence observation the same tissues were further stained by hematoxylin and eosin (HE) and studied comparatively by light microscopy.
The following results were obtained: (1) The normal adult rat brain lacks the cells which react with the antiserum, thus the resting microglia occurring in the normal adult brain are antigenically different from the cells of the monocyte-macrophage system. (2) In the injured brain tissues monocytes extravasate, enter brain parenchyma, and take ameboid forms or become macrophages. (3) Among the reactive cells in the injured brain, all of the brain macrophages and most of the ameboid cells were reactive with the antiserum thereby indicating their monocytic origin.
Similar content being viewed by others
References
Cammermeyer J (1970) The life history of the microglial cell: A light-microscopic study. In: Ehrenpreis S, Solnitzky OC (eds) Neurosciences research, vol 3. Academic Press, New York, pp 44–129
Del Rio Hortega P (1932) Microglia. In: Penfield W (ed) Cytology and cellular pathology of the nervous system, vol 2. Hoeber, New York, pp 481–534
Fujita S, Kitamura T (1976) Origin of brain macrophages and the nature of the microglia. In: Zimmerman HM (ed) Progress in neuropathology, vol 3. Grune & Stratton, New York, pp 1–50
Hager H (1968) Allgemeine morphologische Pathologie des Nervengewebes. In: Handbuch der allgemeinen Pathologie, Bd 2, Die Organe. Springer, Berlin Heidelberg New York, pp 1–385
Kitamura T (1975) Hematogenous cells in experimental Japanese encephalitis. Acta Neuropathol (Berl) 32:341–346
Kitamura T, Hattori H, Fujita S (1972) Autoradiographic studies on histogenesis of brain macrophages in the mouse. J Neuropathol Exp Neurol 31:502–518
Kitamura T, Tsuchihashi Y, Tatebe A, Fujita S (1977) Electronmicroscopic features of the resting microglia in the rabbit hippocampus identified by silver carbonate staining. Acta Neuropathol (Berl) 38:195–201
Kitamura T (1980) Dynamic aspects of glial reactions in altered brains. Pathol Res Pract 168:301–343
Kitamura T, Tsuchihashi Y, Fujita S (1978) Initial response of silverimpregnated “resting microglia” to stab wounding in rabbit hippocampus. Acta Neuropathol (Berl) 44:31–39
Konigsmark BK, Sidman RL (1963) Origin of brain macrophages in the mouse. J Neuropathol Exp Neurol 22:327–328, 642–676
Odashima S (1964) Establishment of ascites hepatomas in the rat. Natl Cancer Inst Monogr 16:51
Oehmichen M (1978) Mononuclear phagocytes in the central nervous system. Springer, Berlin Heidelberg New York
Oehmichen M, Wiethölter H, Greaves MF (1979) Immunological analysis of human microglia: Lack of monocytic and lymphoid membrane diffeentiation antigens. J Neuropathol Exp Neurol 38:99–103
Persson LI, Rönnbäck L, Rosengren LE (1978) Identification of reactive cells in the injured brain. Acta Neurol Scand [Suppl] 67:245–246
Sato M (1968)3H-thymidine autoradiographic studies on the origin of reactive cells in the brain of mice infected with Japanese encephalitis virus (in Japanese). Brain Nerve (Tokyo) 20:1239–1250
Author information
Authors and Affiliations
Additional information
This work was partly supported by a Grant-in-Aid from the Ministry of Education, Science, and Culture, Japan
Rights and permissions
About this article
Cite this article
Tsuchihashi, Y., Kitamura, T. & Fujita, S. Immunofluorescence studies of the monocytes in the injured rat brain. Acta Neuropathol 53, 213–219 (1981). https://doi.org/10.1007/BF00688024
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00688024