Summary
The formation of a blood-brain barrier to horseradish peroxidase was microscopically and ultrastructurally investigated in the tectum opticum of the chick during development of the intraneural blood vessel network from the 6th incubation day to hatching, and in adult specimens.
Extravasation of the circulating marker, apparently unimpeded during early stages of vasculogenesis, starts to diminish from the 14th incubation day (i.d.) and is prevented after the 18th i.d. The tracer seems to get out of the vessel lumina through the sites of reciprocal contact between adjacent endothelial cells, and the differentiation of tight junctions there hinders the passage of peroxidase particles. The formation of numerous endothelial vacuoles during early vasculogenesis and the setting of the blood-brain barrier are discussed in connection with the mechanisms of transendothelial transport, and respectively, the processes of moulding of the growing endothelia.
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References
Bär T (1980) The vascular system of the cerebral cortex. Adv Anat Embryol Cell Biol 59:1–62
Bär T, Wolff JR (1972) The formation of capillary basement membranes during internal vascularization of the rat's cerebral cortex. Z Zellforsch Mikrosk Anat 133:231–248
Bertossi M, Roncali L (1981) Ultrastructural changes of the developing blood vessels in the chick embryo adenohypophysis. J Submicrosc Cytol 13:391–406
Bertossi M, Roncali, L, Ribatti D (1983) Ultrastructural study of the developing blood capillaries in the pineal gland of the domestic fowl. J Submicrosc Cytol 15:965–973
Brightman MW (1977) Morphology of blood-brain interfaces. Exp Eye Res [Suppl] 25:1–25
Brightman MW, Hori M, Rapaport SI, Reese TS, Westergaard E (1973) Osmotic opening of the tight junctions in cerebral endothelium. J Comp Neurol 152:317–325
Brightman MW, Zis K, Anders J (1983) Morphology of cerebral endothelium and astrocytes as determinants of the neuronal microenvironment. Acta Neuropathol (Berl) [Suppl] VIII:21–33
Cervos-Navarro J, Artigas J, Mrsuljia BJ (1983) Morphofunctional aspects of the normal and pathological bloodbrain barrier. Acta Neuropathol (Berl) [Suppl] VIII:1–20
Giacomelli F, Wiener J, Spiro D (1970) The cellular pathology of experimental hypertension. Am J Pathol 59:133–159
Kristensson K, Olsson Y (1973) Accumulation of protein tracers in pericytes of the central nervous system following systemic injection in immature mice. Acta Neurol Scand 49:189–194
Lorenzo AV, Shirahige I, Long M, Barlow CF (1972) Temporary alteration of cerebrovascular permeability to plasma protein. Am J Physiol 22:268–277
Peterson RG (1969) Vascular permeability to horseradish peroxidase in the developing chick brain. Anat Rec 163:341
Reese TS, Karnovsky MJ (1967) Fine structural localization of a blood-brain barrier to exogenous peroxidase. J Cell Biol 34:207–217
Roncali L, Nico B, Ribatti D, Bertossi M, Mancini L (1984a) The blood-brain barrier to horseradish peroxidase in the chick embryo optic tectum developing under normal and hypoxic conditions. 5th International Meeting of the International Society for Developmental Neuroscience [Abstr 29]
Roncali L, Nico B, Ribatti D, Bertossi M, Ambrosi G (1984b) Development of blood-brain barrier to horseradish peroxidase in the tectum opticum of the chick embryo. Int J Microcirc Clin Exp 3:338
Roncali L, Ribatti D, Ambrosi G (1985a) Vasculogenesis in the chick embryo optic tectum. Actanant (Basel) 122:229–234
Roncali L, Ribatti D, Ambrosi G (1985b) Ultrastructural basis of the vessel wall differentiation in the chick embryo optic tectum. J Submicrosc Cytol 17:83–88
Roncali L, Ribatti D, Nico B, Bertossi M (1985c) Blood vessel ultrastructure in the chick embryo optic tectum developing under chronic hypoxia condition. Acta Neuropathol (Berl) 67:242–246
Simionescu M, Simionescu N, Palade GE (1975) Segmental differentiations of cell junctions in the vascular endothelium. The microvasculature. J Cell Biol 67:863–885
Staehelin LA (1974) Structure and function of intercellular junctions. Int Rev Cytol 39:191–278
Van Deurs B (1976) Observations on the blood-brain barrier in hypertensive rats, with particular reference to phagocytic pericytes. Ultrastruct Res 56:65–77
Van Deurs B (1980) Structural aspects of brain barriers, with special reference to the permeability of the cerebral endothelium and choroidal epithelium. Int Rev Cytol 65:117–191
Wakai S, Hirokawa N (1978) Development of the blood-brain barrier to horseradish peroxidase in the chick embryo. Cell Tissue Res 195:195–203
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Roncali, L., Nico, B., Ribatti, D. et al. Microscopical and ultrastructural investigations on the development of the blood-brain barrier in the chick embryo optic tectum. Acta Neuropathol 70, 193–201 (1986). https://doi.org/10.1007/BF00686072
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DOI: https://doi.org/10.1007/BF00686072