Summary
The effects of i.v. infusions of Prostaglandin E2 on renal excretion of sodium and fluid were investigated in rats. Increasing diuresis was observed at infusion rates from 10 to 100 ng/min·100 g BW. Higher rates depressed the arterial blood pressure and urinary excretion rates. At 100 ng PGE2/min·100 g BW urinary flow rate increased approximately 3 times and sodium excretion approximately 8–10 times above the control level. The urine/plasma concentration ratio of Na averaged 1.7 compared with 0.5 in controls. Potassium excretion, however, was only slightly enhanced.
The effects on sodium and fluid excretion were not correlated with changes of total kidney GFR. Single nephron GFR of superficial nephrons remained unchanged during the PGE2 infusion.
Fractional proximal reabsorption of sodium and fluid, calculated from endproximal tubular fluid/plasma concentration ratios of Na and Inulin, were not inflenced by PGE2. Transtubular net movements, calculated from split drop half time, were not significantly affected, too. Tubular transit time, however, was shortened, and fractional proximal reabsorption was decreased, when calculated fromt1/2 andT. This, too, indicates thatt1/2 andT do not always sufficiently define the fractional reabsorption.
The normal decrease of the TF/P ratio of Na along the distal convolution is reduced under the influence of PGE2. Approximately 2% of the filtered Na remain unreabsorbed in the distal convolution in addition to the amount found in controls. The high Na concentration in the final urine is established in the collecting ducts by an ADH like action.
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Fülgraff, G., Meiforth, A. Effects of prostaglandin E2 on excretion and reabsorption of sodium and fluid in rat kidneys (micropuncture studies). Pflugers Arch. 330, 243–256 (1971). https://doi.org/10.1007/BF00588615
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DOI: https://doi.org/10.1007/BF00588615