Summary
The reported experiments demonstrate that in rabbit gallbladder epithelium:
-
a)
The mucosa-cell Na+ and Cl− influxes (45 sec long) are reduced in Cl−-free and Na+-free bathing solutions respectively; transconcentration effects are observed.
-
b)
Cell Cl− labelling through the luminal membrane, with36Cl− (1 hr incubation), is nearly abolished if a Na+-free bathing solution is used; but cell Na+ labelling with22Na+ in the lumen (1 hr incubation) does not change if a Cl−-free bathing solution is employed.
-
c)
Cell Na+ and Cl− labelling through the basolateral membrane (1 hr incubation) is negligible; some Cl− labelling is obtained only when the intracellular electrical potential is artificially reduced.
-
d)
SO 2−4 is found to abolish net water transport, to cross the epithelium, but not to enter the cell.
On the basis of these data a paracellular pathway for net Cl− transport is ruled out, a Na+ and Cl− cotransport through the cell luminal barrier and a Na+ active extrusion through the basolateral membrane are suggested. No definitive conclusion about Cl− extrusion is possible.
Similar content being viewed by others
References
Barry, P. H., Diamond, J. M.: Junction potentials, electrode standard potentials and other problems in interpreting electric properties of membranes. J. Membr. Biol.3, 93–122 (1970)
Barry, P. H., Diamond, J. M., Wright, E. M.: The mechanism of cation permeation in rabbit gallbladder. Dilution potentials and biionic potentials. J. Membr. Biol.4, 358–394 (1971)
Cremaschi, D., Hénin, S., Ferroni, A.: Intracellular electric potential in the epithelial cells of rabbit gallbladder. Bioelectrochem. Bioenergetics1, 208–216 (1974). 2nd Intern. Symp. on Bioclectrochem., Pont à Mousson (1973)
Cremaschi, D., Smith, M. W., Wooding, F. B. P.: Temperature dependent changes in fluid transport across goldfish gallbladder. J. Membr. Biol.13, 143–164 (1973)
Diamond, J. M.: The mechanism of solute transport by the gallbladder. J. Physiol. (Lond.)161, 474–502 (1962)
Diamond, J. M.: Transport of salt and water in rabbit and guinea pig gallbladder: J. gen. Physiol.48, 1–14 (1964a)
Diamond, J. M.: The mechanism of isotonic water transport. J. gen. Physiol.48, 15–42 (1964b)
Dietschy, J. M.: Water and solute movement across the wall of the everted gallbladder. Gastroenterology47, 395–408 (1964)
Dugas, M. C., Frizzell, R. A.: Localization of coupled NaCl transport in rabbit gallbladder. Fed. Proc.33, 370 (1974)
Frömter, E.: The route of passive ion movement through the epithelium of Necturus gallbladder. J. Membr. Biol.8, 259–301 (1972)
Gelarden, R. T., Rose, R. C.: Electrical properties and diffusion potentials in the gallbladder of man, monkey, dog, goose and rabbit. J. Membr. Biol.19, 37–54 (1974)
Hénin, S., Cremaschi, D.: Transcellular ion route in rabbit gallbladder. Electric properties of the epithelial cells. Pflügers Arch.355, 125–139 (1975)
Machen, T. E., Diamond, J. M.: An estimate of the salt concentration in the lateral intercellular spaces of rabbit gallbladder during maximal fluid transport. J. Membr. Biol.1, 194–213 (1969)
Machen, T. E., Erlij, D., Wooding, F. B. P.: Permeable junctional complexes. The movement of Lanthanum across rabbit gallbladder and intestine. J. Cell Biol.54, 302–312 (1972)
Martin, D. W., Diamond, J. M.: Energetics of coupled active transport of sodium and chloride. J. gen. Physiol.50, 295–315 (1966)
Nellans, H. N., Frizzell, R. A., Schultz, S. G.: Coupled sodium chloride influx across the brush border of rabbit ileum. Amer. J. Physiol.225, 467–475 (1973)
Quay, J. F., Armstrong, W.: Sodium and chloride transport by isolated bullfrog small intestine. Amer. J. Physiol.217, 694–702 (1969)
Schultz, S. G., Curran, P. F., Chez, R. A., Fuisz, R. E.: Alanine and sodium fluxes across mucosal border of rabbit ileum. J. gen. Physiol.50, 1241–1260 (1967)
Schultz, S. G., Zalusky, R.: Ion transport in isolated rabbit ileum. I. Shortcircuit current and Na fluxes. J. gen. Physiol.47, 567–584 (1964)
Smulders, A. P., Tormey, J. McD., Wright, E. M.: The effect of osmotically induced water flows on the permeability and ultrastructure of the rabbit gallbladder. J. Membr. Biol.7, 164–197 (1972)
Stein, W. D.: The movement of molecules across cell membranes, pp. 152–157. New York: Academic Press 1967
Van Os, C. H.: Transport parameters of isolated gallbladder epithelium. Doctoral Thesis, Nijmegen (1974)
Wheeler, H. O.: Transport of electrolytes and water across wall of rabbit gallbladder. Amer. J. Physiol.205, 427–438 (1963)
Whitlock, R. T., Wheeler, H. O.: Anion transport by isolated rabbit gallbladders. Amer. J. Physiol.213, 1199–1204 (1967)
Wright, E. M., Diamond, J. M.: Effects of pH and polyvalent cations on the selective permeability of gallbladder epithelium to monovalent ions. Biochim. biophys. Acta (Amst.)163, 57–74 (1968)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cremaschi, D., Hénin, S. Na+ and Cl− transepithelial routes in rabbit gallbladder. Pflugers Arch. 361, 33–41 (1975). https://doi.org/10.1007/BF00587337
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00587337