Abstract
The soleus and gastrocnemius muscles of chronically hypoxic guinea pigs were analyzed for capillary supply and myoglobin concentration. Weanling male guinea pigs were exposed to a simulated altitude of 5,100 m and an average ambient temperature of 22°C for 2, 4, 6, 10 and 14 weeks (range of BW 244–965 g). The soleus and gastrocnemius-plantaris muscles of one leg were analyzed for myoglobin concentration while the soleus and medial head of the gastrocnemius of the contralateral leg were cut at the midpoint, frozen and sectioned in a cryostat. The myosin ATPase method was used to visualize fibers and capillaries. Values of muscle weight, fiber cross sectional area, capillary density, capillary to fiber ratio and the number of capillaries around the fiber were compared to the values of the same parameters from normoxic guinea pigs selected to match the average body weights of the hypoxic animals. The growth rates of the two groups were not different. No significant differences in the regression lines for the normoxic and hypoxic animals were found so that when the data were combined no significant differences in the normoxic lines were introduced by adding the values of the hypoxic animals. The myoglobin values were significantly higher only in the hypoxic soleus after 14 weeks of exposure.
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References
Anthony A, Ackerman E, Strother GK (1959) Effects of altitude acclimatization on rat myoglobin. Changes in myoglobin content of skeletal and cardiac muscle. Am J Physiol 196:512–516
Aquin L, Sillau AH, Lechner AJ, Banchero N (1980) Growth and skeletal muscle microvascularity in the guinea pig. Microvas Res (in press)
Banchero N, Sime F, Penazola D, Cruz J, Gamboa R, Marticorena E (1966) Pulmonary pressure, cardiac output, and arterial oxygen saturation during exercise at high altitude and at sea level. Circulation 33:249–262
Banchero N, Grover RF, Will JA (1971) Oxygen transport in the llama. Respir Physiol 13:102–115
Banchero N, Cruz J, Bustinza J (1975) Mechanisms of O2 transport in Andean dogs. Respir Physiol 23:361–370
Banchero N, Gimenez M, Rostami A, Eby SH (1976) Effects of simulated altitude on O2 transport in dogs. Respir Physiol 27:305–321
Brodal P, Ingjer F, Hermansen L (1977) Capillary supply of skeletal muscle fibers in untrained and endurance trained men. Am J Physiol 232:H705–H712
Cassin SR, Gilbert D, Bunnell CF, Johnson EM (1971) Capillary development during exposure to chronic hypoxia. Am J Physiol 220:448–451
Dubowitz V, Brooke MH (1973) Muscle biopsy: A modern approach. W. B. Saunders, London Philadelphia Toronto
Eby SH, Banchero N (1976) Capillary density of skeletal muscle in Andean dogs. Proc Soc Exp Biol Med 151:795–798
Ellis CA (1972) Effect of endurance running or chronic hypoxia on development of capillaries in rat gastrocnemius muscle. Master Thesis. University of Florida, p 52
Hurtado A, Rotta A, Merino C, Pons J (1937) Studies of myoglobin at high altitudes. Am J Med Sci 194:708–713
Lechner AJ, Banchero N (1980) Lung morphometry in guinea pigs acclimated to hypoxia during growth. Resp Physiol (in press)
Lechner AJ, Salvato VL, Banchero N (1980) Hematology and red cell morphology in guinea pigs acclimated to chronic hypoxia during growth. Comp Biochem Physiol A (in press)
Ingjer F, Brodal P (1978) Capillary supply of skeletal muscle fibers in untrained and endurance trained women. Eur J Appl Physiol 38:291–299
Poel WE (1949) Effect of anoxia on myoglobin concentration in striated muscle. Am J Physiol 156:44–51
Ripoll E, Sillau AH, Banchero N (1979) Changes in the capillarity of skeletal muscle in the growing rat. Pflügers Arch 380:153–158
Sillau AH, Banchero N (1977) Visualization of capillaries in skeletal muscle by the ATPase reaction. Pflügers Arch 369:269–271
Sillau AH, Banchero N (1977a) Effects of hypoxia on capillary density and fiber composition in rat skeletal muscle. Pflügers Arch 370:227–232
Sillau AH, Banchero N (1978) Skeletal muscle fiber size and capillarity. Proc Soc Exp Biol Med 158:288–291
Sillau AH, Banchero N (1979) Effect of hypoxia on the capillarity of guinea pig skeletal muscle. Proc Soc Exp Biol Med 160:368–373
Sillau AH, Aquin L, Lechner AJ, Banchero N (1979) Effects of chronic cold exposure on the capillarity of guinea pig skeletal muscle. Microvas Res 17(3) S24, Abstract
Tappan DV, Reynafarje B (1957) Tissue pigment manifestations of adaptation to high altitude. Am J Physiol 190:99–103
Turek Z, Ringnalda BEM, Graudtner M, Kreuzer F (1973) Myoglobin distribution in the heart of growing rats exposed to a simulated altitude of 3,500 m in their youth or born in the low pressure chamber. Pflügers Arch 340:1–10
Valdivia E (1958) Total capillary bed in striated muscle of guinea pigs native to the Peruvian Mountains. Am J Physiol 194:585–589
Vaughn BE, Pace N (1956) Changes in myoglobin content of the high altitude acclimatized rat. Am J Physiol 185:549–556
Willner LA, Groom AC (1977) Inadequacy of filling the capillary bed of skeletal muscle with microfil. Fed Proc 36:523, Abstract
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Supported by PHS Grant No. HL 18145 from the National Heart, Lung and Blood Institute
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Sillau, A.H., Aquin, L., Bui, M.V. et al. Chronic hypoxia does not affect guinea pig skeletal muscle capillarity. Pflügers Arch. 386, 39–45 (1980). https://doi.org/10.1007/BF00584185
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DOI: https://doi.org/10.1007/BF00584185