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Influence of bile acids on the (Na+−K+)-activated- and Mg2+-activated ATPase of rat colon

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Summary

The influence of various bile acids on the (Na+−K+)-ATPase and Mg2+-ATPase activity of rat colon is described. At a concentration of 0.6 mmol/l C and TC did not inhibit the (Na+−K+)-ATPase activity in contrast to GC. The taurine derivates TC, TCDC and TDC did not influence or even enhanced the (Na+−K+)-ATPase activity. All bile acids except C, TC and CDC depressed the Mg2+-ATPase activity. At higher concentrations only C and TC did not influence the (Na+−K+)-ATPase activity. C, GC and TC at 2.5 mmol/l decreased the (Na+−K+)-activated phosphatase with ATP as substrate. All other substrates tested did not influence the enzymic activity significantly.

The results indicate that bile acids can inhibit the Na+-absorbing system in rat colon. Hence this inhibition can cause diarrhea.

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Abbreviations

C:

cholic acid

GC:

glycocholic acid

TC:

taurocholic acid

CDC:

chenodeoxycholic acid

GCDC:

glycochenodeoxycholic acid

TCDC:

taurochenodeoxycholic acid

DC:

deoxycholic acid

GDC:

glycodeoxycholic acid

TDC:

taurodeoxycholic acid

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Authors and Affiliations

  1. Laboratory for Clinical Chemistry, Department of Internal Medicine, St. Radboud Hospital, University of Nijmegen, Nijmegen, The Netherlands

    J. C. M. Hafkenscheid

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  1. J. C. M. Hafkenscheid
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Hafkenscheid, J.C.M. Influence of bile acids on the (Na+−K+)-activated- and Mg2+-activated ATPase of rat colon. Pflugers Arch. 369, 203–206 (1977). https://doi.org/10.1007/BF00582185

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  • DOI: https://doi.org/10.1007/BF00582185

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