Summary
A yeast (Pichia pastoris strain CMB 10) growing on methanol as sole carbon and energy source was isolated. The high growth rate (0.235·h−1 at pH 5) and high cell yield (0.41 g cell per g methanol at pH 3.5) of this strain are of interest for production of single cell protein (SCP). Other advantages of the strain are: low maintenance coefficient (m=9.5 mg·g−1·h−1), high affinity for methanol (Ks=100 mg·l−1), possibility of non aseptic culture at low pH (pH 3.5), equilibrated amino acid profile and flocculating properties.
Similar content being viewed by others
References
Ballerini D (1978) Procédé IFP de Production de protéines ex-méthanol. Revue Institut Français du Pétrole 33:111–117
Cooney CL, Levine DW (1972) Microbioal utilization of methanol. Adv Appl Microbiol 15:337–365
Conney CL, Levine DW (1974) SCP Production from methanol by yeast in single cell protein II: Tannenbaum S, Wang DC (eds) 402–423
Conney CL, Makiguchi N (1977) An assessement of single cell protein from methanol grown yeast. Biotechnol Bioeng Symp 7:65–76
Couderc R, Baratti J (1980) Oxidation of methanol by the yeast Pichia pastoris IFP 206: Purification and properties of the alcohol oxidase. Agric Biol Chem 44:2279–2289
Hamer G, Pal HS, Hamdan IY (1979) Yield derepression in methanol utilizing batch cultures. Biotechnol Letters 1:9–14
Levine DW, Cooney CL (1973) Isolation and characterization of a thermotolerant methanol utilizing yeast. Appl Microbiol 26:982–990
Minami K, Yamamura M, Shimizu S, Ogawa K, Sekine N (1978) A new methanol assimilating high productive thermophilic yeast. J Ferm Technol 56:1–7
O'Connor ML, Quayle JR (1980) Pentose phosphate-dependant fixation of formaldehyde by methanol grown hansenula polymorpha and Candida boidinii. J Gen Microbiol 26:219–255
Ogata K, Nishikawa H, Ohsugi M, Tochikura T (1970) Studies of the production of yeast. Part II: the cultural conditions of methanol assimilating yeast Klockera sp. n0 2201. J Ferm Technol 48:470–477
Pilat P, Prokop A (1975) The effect of methanol, formaldehyde and formic acid on growth of Candida boidinii 11Bh. Biotechnol Bioeng 17:1717–1728
Sahm H, Wagner F (1972) Mikrobioelle verwertung von methanol; Isolierung und charakterisierung der Hefe Candida boidinii. Arch Mikrobiol 84:29–42
Van Dijken JP, Harder W (1974) Optimal conditions for the enrichement and isolation of methanol assimilating yeast. J Gen Microbiol 84:409–411
Van Dijken JP, Harder W (1975) Growth yields of microorganisms on methanol and methane. A theoretical study. Biotechnol Bioeng 17:15–30
Van Dijken JP, Harder W, Beadsmore AJ, Quayle JR (1978) Dihydroxyacetone: an intermediate in the assimilation of methanol by yeasts? FEMS Microbiol Letters 4:97–102
Held W, Schlanderer G, Reimann J, Dellweg H (1978) Continuous culture of Candida boidinii variant 60 growing on methanol. Eur J Appl Microbiol 6:127–132
Held W, Schlanderer G, Reimann J, Dellweg H (1978) Metabolic changes during substrate shifts in continuous culture of the yeast Candida boidinii variant 60. Eur J Appl Microbiol 6:133–144
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jara, P., Allais, J.J. & Baratti, J. Isolation and characterization of a methanol utilizing yeast with high cell yield. European J. Appl. Microbiol. Biotechnol. 17, 19–23 (1983). https://doi.org/10.1007/BF00510566
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00510566