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Preparation and regeneration of mycelial protoplasts of Collybia veltipes and Pleurotus ostreatus

  • Applied Microbiology
  • Published:
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Summary

The preparation and regeneration of mycelial protoplasts from Collybia veltipes and Pleurotus ostreatus were examined. Composition of liquid medium for cultivation, culture aging of mycelium, and the combination of enzymes had important effects on the isolation of protoplasts. Three characteristic patterns were observed in the regeneration of hyphae from protoplasts.

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Author notes

  1. Osamu Yamada

    Present address: Research Laboratory, the Nisshin Oil Mills, Ltd., Yokohama

  2. Yohichi Kakimoto

    Present address: Nagano Vegetable and Ornamental Crops Experimental Station, Matsushiro, 381-12, Nagano

Authors and Affiliations

  1. National Food Research Institute, P.O. Box 11, 305, Tsakuba Science City, Ibaraki, Japan

    Osamu Yamada, Yumi Magae, Yutaka Kashiwagi, Yohichi Kakimoto & Takashi Sasaki

Authors
  1. Osamu Yamada
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  2. Yumi Magae
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  3. Yutaka Kashiwagi
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  4. Yohichi Kakimoto
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  5. Takashi Sasaki
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Yamada, O., Magae, Y., Kashiwagi, Y. et al. Preparation and regeneration of mycelial protoplasts of Collybia veltipes and Pleurotus ostreatus . European J. Appl. Microbiol. Biotechnol. 17, 298–300 (1983). https://doi.org/10.1007/BF00508024

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  • DOI: https://doi.org/10.1007/BF00508024

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