Summary
Incubation of bovine coronary artery (BCA) rings with isobutylmethylxanthine (IBMX) resulted in a time-dependent increase of cAMP content. This effect was blocked, when the rings were preincubated with indomethacin or 15-hydroperoxy-arachidonic acid for 5 min, indicating that the IBMX-induced increase in cAMP content may depend on endogenous PGI2 formation. PGE2 did not increase the cAMP content in BCA rings. Dipyridamole did not affect cAMP content, when used as a substitute for IBMX. It is suggested that PGI2 stimulates cAMP formation in arterial walls, but that this effect only becomes visible in the presence of a phosphodiesterase inhibitor.
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Dembinska-Kiec, A., Rücker, W. & Schönhöfer, P.S. PGI2 enhanced cAMP content in bovine coronary arteries in the presence of isobutylmethylxanthine. Naunyn-Schmiedeberg's Arch. Pharmacol. 308, 107–110 (1979). https://doi.org/10.1007/BF00499051
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DOI: https://doi.org/10.1007/BF00499051