Summary
For the further clarification of the polymorphism of mouse-esterase and its hormonal control, which in part have not yet been fully comprehended, disc-electrophoretic analyses of eight organs were made, using a strain with the Tfm-mutation. In addition, quantitative assays of esterase activity as well as histochemical studies were performed. The individual organs are characterized by a specific banding pattern of esterase, which is essentially conditioned by the diverse activity of a limited number of bands. Partly these may be regarded as primary gene products, partly they seem to be secondary modifications. The few incidences of band-linkage justify the expectations, that further gene loci will be discovered. In four organs of Tfm-mutants a lower esterase activity was found than in the controls, which was especially distinct in the kidney. The behaviour of the testosterone-dependent bands in the kidneys of Tfm-mutants seems to indicate two different mechanisms of the effect of testesterone on these bands.
Zusammenfassung
Zur weiteren Klärung des teilweise noch unverstandenen Polymorphismus der Mäuseesterase und deren hormoneller Steuerung wurden diskelektrophoretische Analysen von 8 Organen eines Stammes mit Tfm-Mutation durchgeführt und durch quantitative Esterasebestimmungen sowie histochemische Untersuchungen ergänzt. Die Organspezifität der Verteilungsmuster der Esterase ist im wesentlichen durch die unterschiedliche Aktivität einer begrenzten Zahl von Banden bedingt. Diese sind teils als primäre Genprodukte anzusehen, teils verhalten sie sich wie sekundäre Modifikationen. Die geringe Zahl von Bandenkopplungen läßt die Entdeckung weiterer Genloci erwarten. Bei Tfm-Mutanten findet sich in 4 Organen eine niedrigere Esteraseaktivität als in Normaltieren, besonders deutlich in der Niere. Das Verhalten der testosteronabhängigen Esterasebanden der Niere von Tfm-Mutanten spricht für zwei unterschiedliche Mechanismen der Testosteronwirkung auf diese Banden.
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Hermes, B., Riebschläger, M. & Deimling, O.v. Esterase. Histochemistry 43, 81–96 (1975). https://doi.org/10.1007/BF00490157
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DOI: https://doi.org/10.1007/BF00490157