Abstract
Alpha-adrenergic receptor sites in cerebral cortex membranes of the guinea pig brain have been characterized by the specific binding of 3H-WB4101, an alpha-adrenergic antagonist. The binding was rapid, reversible, saturable, and stereoselective. The maximal binding site (B max) and disociation constant (k d) for 3H-WB4101 were calculated to be 251 fmol/mg protein and 1.23 nM, respectively. Ligand displacement experiments suggest that 3H-WB4101 binds to alpha1-adrenergic receptors. Chronic treatment of guinea pigs with desipramine, amitriptyline, phenelzine, or electroconvulsive shock failed to show any statistically significant change in alpha-adrenergic receptor sensitivity as measured by the binding of 3H-WB4101.
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Hu, Hy.Y., Davis, J.M. & Pandey, G.N. Characterization of alpha-adrenergic receptors in guinea pig cerebral cortex: Effect of chronic antidepressant treatments. Psychopharmacology 74, 201–203 (1981). https://doi.org/10.1007/BF00427093
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DOI: https://doi.org/10.1007/BF00427093