Summary
A group of mutants isolated from E. coli K12(tif-1) display a very pleiotropic phenotype. The main characteristic of these mutants, temporarily designated ‘S’ strains, is their ability to suppress a large number of mutations. High efficiency of suppression is correlated with increased thermolability of cellular proteins, indicating an impairment in the fidelity of protein synthesis. Efficient suppression is also accompanied by the appearance of new characteristics like simultaneous resistance to several antibiotic drugs (Sm, Spc, and Mer), and plasmid-like DNA circles. Genetic studies show that the suppressor character is located in the rpoBC region. In a large number of spontaneous Rifr mutants, isolated from these strains, suppression or resistance to drugs is lost. The findings suggest that the phenotype of ‘S’ strains is due to an altered RNA polymerase causing erroneous transcription.
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References
Benzer S, Champe SP (1961) Ambivalent rII mutants of phage T4. Proc Natl Acad Sci USA 47:1025–1038
Biswas DK, Gorini L (1972) Restriction, de-restriction and mistranslation in missense suppression. Ribsomal discrimination of transfer RNAs. J Mol Biol 64:119–134
Bolund C (1973) Phenotypic reversion by 5-fluorouracil of amber mutations in bacteriophage T4: Characterization of rescued functions. Mol Gen Genet 121:247–258
Branscombe EW, Galas DJ (1975) Progressive decrease in protein synthesis accuracy induced by streptomycin in Escherichia coli. Nature 254:161–163
Bujard H, Heidelberger C (1966) Fluorinated pyrimidines XXVII. Attempts to determine transcription errors during the formation of fluorouracil-containing messenger ribonucleic acid. Biochem 5:3339–3351
Chamberlin MJ, Nierman WC, Wiggs J, Neff N (1979) A quantitative assay for bacterial RNA polymerases. J Biol Chem 254:10061–10069
Champe SP, Benzer S (1962) Reversal of mutant phenotypes by 5-fluorouracil: An approach to nucleotide sequences in messenger-RNA. Proc Natl Acad Sci USA 48:532–546
Davies J, Gilbert W, Gorini L (1964) Streptomycin, suppression and the code. Proc Natl Acad Sci USA 51:883–890
Goddard JP, Weiss JJ, Wheeler CM (1969) Error frequency during in vitro transcription of polyU is increased by γ-irradiated RNA polymerase. Nature 222:670–671
Humphreys GO, Willstraw GA, Anderson ES (1975) A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta 383:457–463
Ishihama A, Shimamoto N, Hiroji A, Wakami K, Nashimoto H, Tsugawa A, Uchida H (1980) Temperature-sensitive mutations in the α subunit gene of Escherichia coli RNA polymerase. J. Mol Biol 137:137–150
Langridge J (1968) Thermal responses of mutant enzymes and temperature limits to growth. Mol Gen Genet 103:116–126
Lederberg EM, Cohen SN (1974) Transformation of Salmomella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol 119:1072–1074
Ludlum DB, Wilhelm RC (1968) Ribonucleic acid polymerase reactions with methylated polycytidylic acid templates. J Biol Chem 243:2750–2753
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, New York
Modolell J (1971) The S-30 system from Escherichia coli. Protein biosynthesis in bacterial systems. In: Last Laskin (eds) Methods of Molecular Biology, vol 1. Marcel Dekker, New York
Naono S, Gros F (1960) Synthese par E. coli d'une phosphatase modifié en presence d'un analogue pyrimidinique. CR Acad Sci 250:3889–3891
Nakada D, Magasanik B (1964) The roles of inducer and catabolite repression in the synthesis of β-galactosidase by Escherichia coli. J Mol Biol 8:105–127
Nomura M (1976) Organization of bacterial genes for ribosomal components: Studies using novel approaches. Cell 9:633–644
Old D, Gorini L (1965) Amino acids changes provoked by streptomycin in a polypeptide synthesized in vitro. Science 150:1290–1292
Rosen B (1965) Characteristics of 5-fluorouracil-induced synthesis of alkaline phosphatase. J Mol Biol 11:845–850
Springgate CF, Loeb LA (1975) On the fidelity of transcription by Escherichia coli ribonucleic acid polymerase. J Mol Biol 97:577–591
Strniste GF, Smith DA, Hayes FN (1973) X-ray inactivation of the Escherichia coli deoxyribonucleic acid dependent ribonucleic acid polymerase in aqueous solution. ii. Studies on initiation and fidelity of transcription. Biochem 12:603–608
Volloch VZ, Rits S, Tumernan L (1979) A possible mechanism responsible for the correction of transcription errors. Nucleic Acids Res 6:1535–1546
Witkin EM (1974) Thermal enhancement of ultravilet mutability in a tif-1 uvrA derivative of Escherichia coli B/r: Evidence that ultraviolet mutagenesis depends upon an inducible function. Proc Natl Acad Sci USA 71:1930–1934
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Communicated by E. Bautz
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Ephrati-Elizur, E., Luther-Davies, S. A novel form of suppression due to an altered RNA polymerase. Molec. Gen. Genet. 181, 390–394 (1981). https://doi.org/10.1007/BF00425617
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DOI: https://doi.org/10.1007/BF00425617