Abstract
The formation of R-prime plasmids was selected in crosses involving soybean microsymbionts with genomic Tn5 insertions and carrying plasmid pJB3JI (with one IS2) copy as donors and Escherichia coli HB101 as recipient. Whereas the parent plasmid was 60 kb, recombinant plasmids between 76 kb and 121 kb were obtained. Restriction and Southern analyses confirmed the mobilization of Tn5 on four R-primes from Bradyrhizobium japonicum I-110 and on an R-prime plasmid from Rhizobium fredii HH303. The largest R-prime plasmid was obtained from the rescue of two symbiotically defective R. fredii mutant strains that required adenosine.
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Abbreviations
- TDP:
-
transposon donor pool
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Scientific article number A-4728 and contribution number 7724 of the Maryland Agricultural Experiment Station
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Shah, K.S., David Kuykendall, L. & Kim, CH. R-prime plasmids from Bradyrhizobium japonicum and Rhizobium fredii . Arch. Microbiol. 152, 550–555 (1989). https://doi.org/10.1007/BF00425485
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DOI: https://doi.org/10.1007/BF00425485