Summary
An EcoR1 shotgun of Yarrowia lipolytica DNA was inserted into the plasmid YIp333 which carries the LYS2 gene of S. cerevisiae. The resulting plasmid pool was transformed in both S. cerevisiae and Y. lipolytica. Whereas numerous replicating plasmids could be isolated from the S. cerevisiae Lys+ transformants, all transformants of Y. lipolytica so far analyzed were found to result from integrative transformation. This occurred at a frequency of 1 to 10 transformants per μg of input DNA. Co-transformation occurred at high frequency and resulted in tandem integration of 2 to 10 copies of the incoming DNA. Structural and segregational stability of the transforming DNA were both high.
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References
Bassel JB, Mortimer RK (1982) Curr Genet 5:77–88
Beach D, Nurse P (1981) Nature (London) 290:140–142
Beckerich JM, Colonna Ceccaldi B, Lambert M, Heslot H (1984) Curr Genet 8:531–536
Beggs JD (1978) Nature (London) 275:104–109
Das S, Hollenberg CP (1982) Curr Genet 6:123–126
Davidow LS, Apostolakos, D, O'Donnell MM, Proctor AR, Ogrydziak DM, Wing RA, Stasko I, DeZeeuw JR (1985) Curr Genet 10:39–48
Davis RW, Botstein D, Roth JR (1980) Advanced bacterial genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 116–217
Eibel H, Philippsen P (1983) Mol Gen Genet 191:66–73
Gaillardin CM, Charoy V, Heslot H (1973) Arch Microbiol 92:69–83
Gaillardin CM, Poirier L, Ribet AM, Heslot H (1979) Biochimie 61:473–482
Heslot H, Gaillardin CM, Beckerich JM, Fournier P (1979) Control of lysine metabolism in the petroleum yeast Saccharomycopsis lipolytica. In: Sebek OK, Laskin A (eds) Genetics of industrial microorganisms. American Society for Microbiology, Washington, DC, pp 54–60
Ito H, Fukuda Y, Murata K, Kimura A (1983) J Bacteriol 153:163–168
Jimenez A, Davies J (1980) Nature (London) 287:869–871
Losson R, Lacroute F (1983) Cell 32:371–377
de Louvencourt L, Fukuhara H, Heslot H, Wesolowski M (1983) J Bacteriol 154:737–742
Murray K, Szostak JW (1983) Cell 34:961–970
Ogrydziak DM, Sharf SS (1982) J Gen Microbiol 128:1225–1234
Ogrydziak DM, Bassel J, Mortimer RK (1982) Mol Gen Genet 188:179–183
Sakaguchi J, Yamamoto M (1982) Proc Natl Acad Sci USA 79:7819–7823
Sherman F, Fink GR, Hicks JB (1979) Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 90–92
Struhl K, Stinchcomb DT, Scherer S, Davis RW (1979) Proc Natl Acad Sci USA 76:1035–1039
Wickerham LJ, Kurtzman CP, Herman AL (1969) Sexuality in Candida lipolytica. In: Ahearn G (ed) Recent trends in yeast research, vol 1. Spectrum, Georgia State University, Atlanta, pp 81–92
Zamir A, Maina CV, Fink GR, Szalay AA (1981) Proc Natl Acad Sci USA 78:3496–3500
Zukowski MM, Gaffner DF, Speck D, Kauffmann M, Findeli A, Wisecup A, Lecoq JP (1983) Proc Natl Acad Sci USA 80:1101–1105
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Gaillardin, C., Ribet, A.M. & Heslot, H. Integrative transformation of the yeast Yarrowia lipolytica . Curr Genet 10, 49–58 (1985). https://doi.org/10.1007/BF00418493
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DOI: https://doi.org/10.1007/BF00418493