Abstract
The recent introduction of 31P nuclear magnetic resonance spectroscopy offers a new approach to the problem of obtaining a simultaneous and direct evaluation of both the cytoplasmic and vacuolar pH in higher plant cells (J. K. M. Roberts, P.M. Ray, N. Waderlardetzky and O. Sardetzky, 1980, Nature 283, 870–872; 1981, Planta 152, 74–78). Using this method we have been able to detect a selective pH decrease of about 0.5 units at the level of the cytoplasmic compartment of maize root tips when the temperature was increased from 4 to 28°C. This effect was completely reversible with temperature. No pH variation could be detected at the level of the vacuolar compartment.
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Aducci, P., Federico, R., Carpinelli, G. et al. Temperature dependence of intracellular pH in higher plant cells. Planta 156, 579–582 (1982). https://doi.org/10.1007/BF00392784
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DOI: https://doi.org/10.1007/BF00392784