Abstract
31P nuclear magnetic resonance (NMR) is a unique technique to monitor noninvasively the energetics of living systems at real time through the detection of a variety of phosphorylated metabolites. Using adequately designed α-aminophosphonates as external probes, we have shown earlier that 31P NMR can also give access simultaneously to the accurate pH of cytosolic and acidic compartments in normal and stressed cultured cells or isolated perfused organs, a feature that was not possible using endogenous inorganic phosphate as the probe. More recently, we obtained a series of derivatives of these new pH probes that incorporate a triphenylphosphonium cation as a specific vector to the mitochondrion. Here, we describe the synthesis, 31P NMR pH titrating properties in buffers, and application in cultures of the green alga Chlamydomonas reinhardtii of two of these mitochondria-targeted pH probes in comparison with one nonvectorized, yet still informative α-aminophosphonate.
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Acknowledgements
This work was supported by grants from the CNRS and Aix-Marseille Université, and by ANR grants (ANR–09-BLAN-005-03). The authors thank A. Krieger-Liszkay for discussion and G. Herbette (Spectropole, Aix-Marseille Université) for his help in NMR recording.
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Culcasi, M., Thétiot-Laurent, S., Atteia, A., Pietri, S. (2015). Mitochondrial, Acidic, and Cytosolic pHs Determination by 31P NMR Spectroscopy: Design of New Sensitive Targeted pH Probes. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 1265. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2288-8_11
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DOI: https://doi.org/10.1007/978-1-4939-2288-8_11
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