Summary
Morphology and distribution of the relatively less well known organelles of plants have been studied with the electron microscope in tissues fixed in glutaraldehyde and postfixed in osmium tetroxide. An organelle comparable morphologically to the animal microbody and similar to the plant microbody isolated by Mollenhauer et al. (1966) has been encountered in a variety of plant species and tissues, and has been studied particularly in bean and radish roots, oat coleoptiles, and tobacco roots, stems and callus. The organelle has variable shape and is 0.5 to 1.5 μ in the greatest diameter. It has a single bounding membrane, a granular to fibrillar matrix of variable electron density, and an intimate association with one or two cisternae of rough endoplasmic reticulum (ER). Microbodies are easily the most common and generally distributed of the less well characterized organelles of plant cells. It seems very probable that they contain the enzymes characteristic of animal lysosomes (containing hydrolases) or animal microbodies (containing catalase and certain oxidases). Spherosomes are also possible sites of enzyme activity but are not as common or as widely distributed as microbodies. For this reason it appears likely that the particles designated as “plant lysosomes”, “spherosomes”, “peroxisomes”, etc., in some of the cytochemical and biochemical studies on enzyme localization will prove to be microbodies.
Variations in the morphology and ER associations of microbodies in tissues of bean and radish are described and discussed. “Crystal-containing bodies” (CCBs) are interpreted as a specialized type of microbody characteristic of metabolically less active cells. Stages in the formation of CCBs from microbodies of typical appearance are illustrated for Avena.
The general occurrence of microbodies in meristematic and differentiating cells and their close association with the ER suggest that they may play active roles in cellular metabolism. The alterations in their morphology and numbers that are observed in certain differentiating cells suggest further that the enzyme complements and metabolic roles of microbodies might change during cellular differentiation. If so, microbodies could be the functional equivalent of both microbodies and lysosomes of animal cells.
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References
Arnott, H. J., and K. M. Smith: Flectron microscopy of virus-infected sunflower leaves. J. Ultrastruct. Res. 19, 173–195 (1967).
Avers, C. J.: Fine structure of Phleum root meristem cells. I. Mitochondria. Amer. J. Bot. 49, 996–1003 (1962).
Balz, H. P.: Intrazelluläre Lokalisation und Funktion von hydrolytischen Enzymen bei Tabak. Planta (Berl.) 70, 207–236 (1966).
Bonnett, H. T., and E. H. Newcomb: Polyribosomes and cisternal accumulations in root cells of radish. J. Cell Biol. 27, 423–432 (1965).
Bouck, G. B.: Fine structure and organelle associations in brown algae. J. Cell Biol. 26, 523–537 (1965).
—, and J. Cronshaw: The fine structure of differentiating sieve tube elements. J. Cell Biol. 25, 79–96 (1965).
Cronshaw, J.: Crystal containing bodies of plant cells. Protoplasma (Wien) 59, 318–325 (1964).
—, and G. B. Bouck: The fine structure of differentiating xylem elements. J. Cell Biol. 24, 415–431 (1965).
Daems, W. T.: The fine structure of mouse-liver microbodies. J. Microscopie 5, 295–304 (1966).
Duve, C. de, and P. Baudhuin: Peroxisomes (Microbodies and related particles). Physiol. Rev. 46, 323–357 (1966).
—, and R. Wattiaux: Functions of lysosomes. Ann. Rev. Physiol. 28, 435–492 (1966).
Essner, E.: Endoplasmic reticulum and the origin of microbodies in fetal mouse liver. Lab. Invest. 17, 71–87 (1967).
Frey-Wyssling, A., E. Grieshaber, and K. Mühlethaler: Origin of spherosomes in plant cells. J. Ultrastruct. Res. 8, 506–516 (1963).
Gahan, P. B.: Histochemical evidence for the presence of lysosome-like particles in root meristem cells of Vicia faba. J. exp. Bot. 16, 350–355 (1965).
—, and A. J. Maple: The behavior of lysosome-like particles during cell differentiation. J. exp. Bot. 17, 151–155 (1966).
Gerola, F. M., and M. Bassi: Sui cristalloidi proteici delle cellule vegetali. Caryologia 17, 399–407 (1964).
Harrington, J. F., and A. M. Altschul: Lysosome-like behavior in germinating onion seeds. (Abstr.) Fed. Proc. 22, 475 (1963).
Holcomb, G. E., A. C. Hildebrandt, and R. F. Evert: Staining and acid phosphatase reactions of spherosomes in plant tissue culture cells. Amer. J. Bot. 54, 1204–1209 (1967).
Hruban, Z., H. Swift, and R. W. Wissler: Alterations in the fine structure of hepatocytes produced by β-3-thienylalanine. J. Ultrastruct. Res. 8, 236–250 (1963).
Jacks, T. J., L. Y. Yatsu, and A. M. Altschul: Isolation and characterization of peanut spherosomes. Plant Physiol. 42, 585–597 (1967).
Jensen, T. E., and J. G. Valdovinos: Fine structure of abscission zones. I. Abscission zones of the pedicels of tobacco and tomato flowers at anthesis. Planta (Berl.) 77, 298–318 (1967).
Kolehmainen, L., H. Zech, and D. von Wettstein: The structure of cells during tobacco mosaic virus reproduction. II. Mesophyll cells containing virus crystals. J. Cell Biol. 25, 77–98 (1965).
Manton, I.: Observations on phragmosomes. J. exp. Bot. 12, 108–113 (1961).
Marinos, N. G.: Comments on the nature of a crystal-containing body in plant cells. Protoplasma (Wien) 60, 31–33 (1965).
Matile, P., J. P. Balz, E. Semadeni, and M. Jost: Isolation of spherosomes with lysosome characteristics from seedlings. Z. Naturforsch. 20b, 693–698 (1965).
Mollenhauer, H. H.: Plastic embedding mixtures for use in electron microscopy. Stain Technol. 39, 111–114 (1964).
—, D. J. Morré, and A. G. Kelley: The widespread occurrence of plant cytosomes resembling animal microbodies. Protoplasma (Wien) 62, 44–52 (1966).
Novikoff, A. B., and W., Shin: The endoplasmic reticulum in the Golgi zone and its relation to microbodies, Golgi apparatus and autophagic vacuoles in rat liver cells. J. Microscopie 3, 187–206 (1964).
O'Brien, T. P., and K. V. Thimann: Observations on the fine structure of the oat coleoptile. II. The parenchyma cells of the apex. Protoplasma (Wien) 63, 417–442 (1967).
Olszewska, M. J., et B. Gabara: Recherches cytochimiques sur la présence de certaines hydrolases au cours de la cytocinèse chez les plantes supérieures. Protoplasma (Wien) 59, 164–179 (1964).
Petzold, H.: Kristalloide Einschlüsse in Zytoplasma pflanzlicher Zellen. Protoplasma (Wien) 64, 120–133 (1967).
Plesnicar, M., W. D. Bonner, Jr., and B. T. Storey: Peroxidase associated with higher plant mitochondria. Plant Physiol. 42, 366–370 (1967).
Porter, K. R., and J. B. Caulfield: The formation of the cell plate during cytokinesis in Allium cepa L. Proc. IVth Internat. Conf. Electron Microscopy (Berlin) 2, 503–509 (1958).
—, and R. D. Machado: Studies on the endoplasmic reticulum. IV. Its form and distribution during mitosis in cells of onion root tip. J. biophys. biochem. Cytol. 7, 167–180 (1960).
Poux, N.: Ultrastructural localization of aryl sulfatase activity in plant meristematic cells. J. Histochem. Cytochem. 14, 932–933 (1966).
Price, W. C.: Flexulous rods in phloem cells of line plants infected with citrus tristeza virus. Virology 29, 285–294 (1966).
Rasch, E. M., C. Kanjiraparamban, and W. F. Millington: Histochemical localization of acid phosphatases in differentiating and necrotic plant cells. J. Cell Biol. 27, 142 A (1965).
Rhodin, J.: Correlation of ultrastructural organization and function in normal and experimentally changed proximal convoluted tubule cells of the mouse kidney. Stockholm: A. B. Godvie 1954.
Schnepf, E.: Zur Cytologie und Physiologie der pflanzlichen Drüsen. IV. Licht-und elektronenmikroskopische Untersuchungen an Septalnektarien. Protoplasma (Wien) 58, 137–171 (1964).
Semadeni, E. G.: Enzymatische charakterisierung der Lysosomenäquivalente (Sphärosomen) von Maiskeimlingen. Planta (Berl.) 72, 91–118 (1967).
Sievers, A.: Lysosomen-ähnliche Kompartimente in Pflanzenzellen. Naturwissenschaften 53, 334–335 (1966).
—: Elektronenmikroskopische Untersuchungen zur geotropischen Reaktion. II. Die polare Organisation des normal wachsenden Rhizoids von Chara joetida. Protoplasma (Wien) 64, 225–253 (1967).
Sorokin, H. P., and S. Sorokin: The spherosomes of Campanula persicifolia L. Protoplasma (Wien) 62, 216–236 (1966).
Thornton, R. M., and K. V. Thimann: On a crystal-containing body in cells of the oat coleoptile. J. Cell Biol. 20, 345–350 (1964).
Tolbert, N. E., A. Oeser, T. Kisaki, R. H. Hageman, and R. K. Yamazaki: Peroxisomes from leaves with enzymes related to glycolate metabolism. (Abstr.) Fed. Proc. 27, 344 (1968).
Villiers, T. A.: Crystalloid structure in the microbodies of plant embryo cells. Life Sci. 6, 2151–2156 (1967).
Walek-Czernecka, A.: Histochemical demonstration of some hydrolytic enzymes in the spherosomes of plant cells. Acta Soc. Bot. Polon. 34, 573–588 (1965).
Walles, B.: Plastid structures of carotenoid-deficient mutants of sunflower (Helianthus annuus L.). I. The white mutant. Hereditas (Lund.) 53, 247–256 (1965).
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NASA Predoctoral Trainee.
Public Health Service Postdoctoral Fellow.
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Frederick, S.E., Newcomb, E.H., Vigil, E.L. et al. Fine-structural characterization of plant microbodies. Planta 81, 229–252 (1968). https://doi.org/10.1007/BF00391159
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DOI: https://doi.org/10.1007/BF00391159