Abstract
Similar to land plant cells, brown algal cells possess plasmodesmata with minute cytoplasmic tunnels, which enable the direct connection between adjacent cells. Plasmodesmata are distributed depending on the association of their formation with cytokinesis. Primary plasmodesmata are formed during cytokinesis, while secondary plasmodesmata appear on the cell wall septum following cytokinesis. Typically, the plasmodesmata of brown algae are cylindrical without the penetration of desmotubules from the endoplasmic reticulum, and there are no morphological differences between primary and secondary plasmodesmata. This present chapter describes the observation of cytokinesis and primary plasmodesmata formation in brown algae using electron microscopy as well as the examination of polysaccharide distribution using antibodies and enzyme-gold probes.
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Acknowledgments
The authors are grateful to Dr. Toshiaki Ito (Electron Microscope Laboratory, Research Faculty of Agriculture, Hokkaido University) for supporting this research. This work was supported by JSPS KAKENHI [grant numbers JP 26440160 and JP 17 K07462].
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Nagasato, C., Yonamine, R., Motomura, T. (2022). Ultrastructural Observation of Cytokinesis and Plasmodesmata Formation in Brown Algae. In: Caillaud, MC. (eds) Plant Cell Division. Methods in Molecular Biology, vol 2382. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1744-1_16
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DOI: https://doi.org/10.1007/978-1-0716-1744-1_16
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