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Sodium efflux from perfused giant algal cells

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Abstract

Internodal cells of the giant alga Chara corallina were perfused internally to replace the native cytoplasm, tonoplast and vacuole with artificial cytoplasm. Sodium efflux from perfused cells, measured by including 22Na in the perfusion media, was increased by increasing the internal sodium concentration and by decreasing the external pH, and was inhibited by external application of the renal diuretic amiloride. The sodium efflux was markedly ATP-dependent, with a 50-fold decrease in efflux observed after perfusion with media lacking ATP. Efflux in the presence of ATP was reduced by 33% by inclusion of 10 μM N,N′-dicyclohexylcarbodiimide in the perfusion medium. The membrane potential of the perfused cells approximated that of intact cells from the same culture. It is suggested that sodium efflux in perfused Chara cells proceeds via a secondary antiporter with protons, regulated by ATP in a catalytic role and with the proton motive force acting as the energy source.

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Abbreviations

DCCD:

N,N′-dicyclohexylcarbodiimide

EGTA:

ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid

Mes:

2-(N-morpholino)ethanesulphonic acid

Mops:

3(N-morpholino)propanesulphonic acid

Taps:

tris(hydroxymethyl)methylaminopropanesulphonic acid

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  1. Botany Department, University of Cambridge, Downing Street, CB2 3EA, Cambridge, UK

    G. M. Clint & E. A. C. MacRobbie

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  1. G. M. Clint
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  2. E. A. C. MacRobbie
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Clint, G.M., MacRobbie, E.A.C. Sodium efflux from perfused giant algal cells. Planta 171, 247–253 (1987). https://doi.org/10.1007/BF00391101

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  • DOI: https://doi.org/10.1007/BF00391101

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