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Kinetics of escherichia coli hydrogen production during short term repeated aerobic-anaerobic fluctuations

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Abstract

A coupled reactor system, consisting of one aerated stirred reactor and one anaerobic plug flow reactor was constructed. Circulation of microbial cells in this system, is chosen to represent the insufficient mixing conditions, present in a large scale vessel. Hereby it is shown, that oscillating oxygen concentrations, give effects on E.coli metabolism. The hydrogen production, resulting from mixed acid fermentation of anaerobically grown cells, is used to indicate the metabolic changes. These changes are measured as hydrogen concentration in the gas outlet of the aerated fermenter, with a Pd-MOS sensor. The dependency of the hydrogen evolution on the anaerobic residence time is shown, and the relevance of this model system for studies on the bioreactor performance, are discussed.

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Authors and Affiliations

  1. Dept. of Biochemistry and Biotechnology, Royal Institute of Technology, S-100 44, Stockholm, Sweden

    G. Larsson & S. -O. Enfors

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  1. G. Larsson
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  2. S. -O. Enfors
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Larsson, G., Enfors, S.O. Kinetics of escherichia coli hydrogen production during short term repeated aerobic-anaerobic fluctuations. Bioprocess Engineering 9, 167–172 (1993). https://doi.org/10.1007/BF00389925

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