Summary
The effects of sodium chloride (NaCl) and abscisic acid (ABA) on protein synthesis, protein hydrolysis, glutamine synthetase, Δ-pyrroline-5-carboxylate reductase and amino acid composition have been studied in aerial parts of Pennisetum typhoides (Stapf et Hubbard) seedlings. Whereas protein synthesis is inhibited by NaCl and ABA, the activity or the synthesis of leucine-arylamidase, glutamine synthetase and Δ-pyrroline-5-carboxylate reductase is promoted by both the substances. The results of the amino acid analysis showed that in the presence of NaCl and ABA the amounts of alanine, aspartate and ammonia decreased, while the concentrations of glutamate, and especially that of proline increased greatly. The possible significance of the results in explaining the proline accumulation under stress conditions and ABA-treatment is discussed.
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Abbreviations
- ABA:
-
Abscisinsäure
Literatur
Addicott, F. T.: Biochemical aspects of abscisic acid. In: Plant growth substances 1970, D. J. Carr, ed., p. 272–280. Berlin-Heidelberg-New York: Springer 1972
Addicott, F. T., Lyon, J. L.: Physiology of abscisic acid and related substances. Ann. Rev. Plant Physiol. 20, 139–164 (1969)
Aspinall, D., Singh, N. T., Paleg, L. G.: Stress metabolism. V. Abscisic acid and nitrogen metabolism in barley and Lolium temulentum L. Aust. J. biol. Sci. 26, 319–327 (1973)
Barnett, N. M., Naylor, A. W.: Amino acid and protein metabolism in Bermuda grass during water stress. Plant Physiol. 41, 1222–1230 (1966)
Bergmeyer, H. U. (Ed.): Methoden der enzymatischen Analyse. Weinheim: Verlag Chemie 1970
Hsiao, T. C.: Plant responses to water stress. Ann. Rev. Plant Physiol. 24, 519–570 (1973)
Huber, W., Sankhla, N.: Eco-physiological studies on Indian arid zone plants. II. Effects of salinity and gibberellin on the activity of the enzymes of amino-acid metabolism in leaves of Pennisetum typhoides. Oecologia 13, 271–277 (1973)
Huber, W., Rustagi, P. N., Sankhla, N.: Eco-physiological studies on Indian arid zone plants. III. Effect of sodium chloride and Gibberellin on the activity of the enzymes of carbohydrate metabolism in leaves of Pennisetum typhoides. Oecologia 15, 77–86 (1974)
Huber, W., Sankhla, N.: Untersuchungen über den Einfluß von Abscisin- und Gibberellinsäurebehandlung auf die Aktivitäten einiger Enzyme des Kohlenhydratstoffwechsels in Blättern von Pennisetum typhoides Keimlingen. Planta (Berl.) 116, 55–64 (1974)
Jones, T. W. A., Stoddart, J. L.: Gibberellin induced changes in protein synthesis and enzyme activity in shoot apices of Trifolium pratense. J. exp. Bot. 21, 452–461 (1970)
Kahane, J., Poljakoff-Mayber, A.: Effects of substrate salinity on the ability for protein synthesis in pea roots. Plant Physiol. 43, 1115–1119 (1968)
Levitt, J.: Responses of plants to environmental stresses. New York-London: Academic Press 1972
Loomis, W. D.: Amide metabolism in higher plants. III. Distribution of glutamyl-transferase and glutamine synthetase activity. Plant Physiol. 34, 541–546 (1959)
Mazelis, M., Creveling, R. K.: L-Proline dehydrogenase of Triticum vulgare germ: Purification, properties and cofactor interactions. Phytochemistry 13, 559–565 (1974)
Mazelis, M., Fowden, L.: Conversion of ornithine into proline by enzymes from germinating peanut cotyledons. Phytochemistry 8, 801–809 (1969)
Mazelis, M., Fowden, L.: The metabolism of proline in higher plants. II. L-proline dehydrogenase from cotyledons of germinating peanut (Arachis hypogea L.) seedlings. J. exp. Bot. 22, 137–145 (1971)
Mizrahi, Y., Blumenfeld, A., Bittner, S., Richmond, A. E.: Abscisic acid and cytokinin content of leaves in relation to salinity and relative humidity. Plant Physiol. 48, 752–755 (1971)
Mizrahi, Y., Blumenfeld, A., Richmond, A. E.: Abscisic acid and transpiration in leaves in relation to osmotic root stress. Plant Physiol. 46, 169–171 (1970)
Mizrahi, Y., Blumenfeld, A., Richmond, A. E.: The role of abscisic acid and salination in the adaptive response of plants to reduced root aeration. Plant & Cell Physiol. 13, 15–21 (1972)
Morris, C. J., Thompson, J. F., Johnson, C. M.: Metabolism of glutamic acid and N-acetylglutamic acid in leaf discs and cell free extracts of higher plants. Plant Physiol. 44, 1023–1026 (1969)
Palfi, G.: Changes in the amino acid content of detached wilting leaves of Solanum laciniatum Ait. in the light and in the dark. Acta Agron. Acad. Sci. Hung. 17, 381–388 (1968)
Prisco, J. T., O'Leary, J. W.: Effect of salt and water stresses on protein synthesizing capacity of embryo-axis of germinating Phaseolus vulgaris L. seeds. Rev. Brasil. Biol. 36, 317–321 (1970)
Sankhla, N., Huber, W.: Enzyme activities in Pennisetum seedlings germinated in the presence of abscisic and gibberelic acids. Phytochemistry 13, 543–546 (1974a)
Sankhla, N., Huber, W.: Effect of abscisic acid on the activities of photosynthetic enzymes and 14CO2-fixation products in leaves of Pennisetum typhoides seedlings. Physiol. Plant. 30, 291–294 (1974b)
Sankhla, N., Huber, W.: Eco-physiological studies on Indian arid zone plants. IV. Effect of salinity and gibberellin on the activities of photosynthetic enzymes and 14CO2-fixation products in leaves of Pennisetum typhoides seedlings. Bioch. Physiol. Pflanzen (im Druck) (1974c)
Singh, T. N., Paleg, L. G., Aspinall, D.: Stress metabolism I. Nitrogen metabolism and growth in the barley plant during water stress. Aust. J. biol. Sci. 26, 45–56 (1973)
Splittstoesser, S. A., Splittstoesser, W. E.: Pyrroline-5-carboxylate reductase from Cucurbita cotyledons. Phytochemistry 12, 1565–1568 (1973)
Strecker, H. J.: The preparation of animal proline oxidase (Rat liver), and its use for the preparation of Δ-Pyrroline-5-carboxylate. In: Methods of enzymology, Tabor, H., Tabor, C. W., eds., vol. 17 B, p. 251–252. New York: Academic Press 1971
Waisel, Y.: Biology of halophytes. London: Academic Press 1972
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Huber, W. Über den Einfluß von NaCl- oder Abscisinsäurebehandlung auf den Proteinmetabolismus und einige weitere Enzyme des Aminosäurestoffwechsels in Keimlingen von Pennisetum typhoides . Planta 121, 225–235 (1974). https://doi.org/10.1007/BF00389323
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DOI: https://doi.org/10.1007/BF00389323